BMRB Entry 27482

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27482

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Title:
Amide backbone chemical shift assignments for SET nuclear proto-oncogene.
Deposition date:
2018-05-15
Original release date:
2019-04-12
Authors:
Parnham, Stuart; De Palma, Ryan; Ogretmen, Besim
Citation:

Citation: De Palma, Ryan; Parnham, Stuart; Li, Yitong; Oaks, Joshua; Peterson, Yuri; Szulc, Zdzislaw; Roth, Braden; Xing, Yongna; Ogretmen, Besim. "The NMR-based characterization of the FTY720-SET complex reveals an alternative mechanism for the attenuation of the inhibitory SET-PP2A interaction"  FASEB J. 33, 7647-7666 (2019).
PubMed: 30917007

Assembly members:

Assembly members:
NdCd-SET_nuclear_proto-oncogene, polymer, 205 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NdCd-SET_nuclear_proto-oncogene: GSMTSEKEQQEAIEHIDEVQ NEIDRLNEQASEEILKVEQK YNKLRQPFFQKRSELIAKIP NFWVTTFVNHPQVSALLGEE DEEALHYLTRVEVTEFEDIK SGYRIDFYFDENPYFENKVL SKEFHLNESGDPSSKSTEIK WKSGKDLTKRSSQTQNKASR KRQHEEPESFFTWFTDHSDA GADELGEVIKDDIWPNPLQY YLVPD

Data sets:
Data typeCount
13C chemical shifts106
15N chemical shifts92
1H chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NdCd-SET, subunit 11
2NdCd-SET, subunit 21

Entities:

Entity 1, NdCd-SET, subunit 1 205 residues - Formula weight is not available

Residues GSM (-3, -2, -1)

1   GLYSERMETTHRSERGLULYSGLUGLNGLN
2   GLUALAILEGLUHISILEASPGLUVALGLN
3   ASNGLUILEASPARGLEUASNGLUGLNALA
4   SERGLUGLUILELEULYSVALGLUGLNLYS
5   TYRASNLYSLEUARGGLNPROPHEPHEGLN
6   LYSARGSERGLULEUILEALALYSILEPRO
7   ASNPHETRPVALTHRTHRPHEVALASNHIS
8   PROGLNVALSERALALEULEUGLYGLUGLU
9   ASPGLUGLUALALEUHISTYRLEUTHRARG
10   VALGLUVALTHRGLUPHEGLUASPILELYS
11   SERGLYTYRARGILEASPPHETYRPHEASP
12   GLUASNPROTYRPHEGLUASNLYSVALLEU
13   SERLYSGLUPHEHISLEUASNGLUSERGLY
14   ASPPROSERSERLYSSERTHRGLUILELYS
15   TRPLYSSERGLYLYSASPLEUTHRLYSARG
16   SERSERGLNTHRGLNASNLYSALASERARG
17   LYSARGGLNHISGLUGLUPROGLUSERPHE
18   PHETHRTRPPHETHRASPHISSERASPALA
19   GLYALAASPGLULEUGLYGLUVALILELYS
20   ASPASPILETRPPROASNPROLEUGLNTYR
21   TYRLEUVALPROASP

Samples:

sample_1: NdCd-SET nuclear proto-oncogene, [U-100% 13C; U-100% 15N; U-80% 2H], 0.4 ± 0.2 mM; NdCd-SET nuclear proto-oncogene, [U-100% 13C; U-100% 15N], 0.5 ± 0.2 mM; NdCd-SET nuclear proto-oncogene, [U-100% 15N], 1.0 ± 0.2 mM; phosphate 20 mM; naCl 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 8.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr v2.4, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks