BMRB Entry 27475

Name: NMR assignments of the mammalian proteasome subunit DSS1.
Published: 2019-05-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27475

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignments of the mammalian proteasome subunit DSS1.

Deposition date:

2018-05-09

Original release date:

2019-05-10

Authors:

Yu, Liping; Hengel, Sarah; Spies, Maria

Citation:

Citation: Hengel, Sarah; Yu, Liping; Spies, Maria. "NMR assignments of the mammalian proteasome subunit DSS1." Nat. Commun. ., .-..

Assembly members:

Assembly members:
DSS1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-KG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DSS1: GSPGISGGGGGILDSMGMSE KKQPVDLGLLEEDDEFEEFP AEDWAGLDEDEDAHVWEDNW DDDNVEDDFSNQLRAELEKH GYKMETS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	244
15N chemical shifts	81
1H chemical shifts	81

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DSS1 monomer	1

Entities:

Entity 1, DSS1 monomer 87 residues - Formula weight is not available

1

GLY

SER

PRO

GLY

ILE

SER

GLY

2

GLY

ILE

LEU

ASP

SER

MET

GLY

MET

SER

GLU

3

LYS

GLN

PRO

VAL

ASP

LEU

GLY

LEU

4

GLU

ASP

GLU

PHE

GLU

PHE

PRO

5

ALA

GLU

ASP

TRP

ALA

GLY

LEU

ASP

GLU

ASP

6

GLU

ASP

ALA

HIS

VAL

TRP

GLU

ASP

ASN

TRP

7

ASP

ASN

VAL

GLU

ASP

PHE

SER

8

ASN

GLN

LEU

ARG

ALA

GLU

LEU

GLU

LYS

HIS

9

GLY

TYR

LYS

MET

GLU

THR

SER

Samples:

sample_1: DSS1, [U-100% 13C; U-100% 15N], 0.22 mM; TRIS 25 mM; sodium chloride 50 mM; EDTA 25 uM; DTT 2 mM

sample_conditions_1: ionic strength: 75 mM; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks