BMRB Entry 27471

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27471

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Title:
peptide AapA1 toxin of Helicobacter pylori
Deposition date:
2018-05-03
Original release date:
2020-02-28
Authors:
Korkut, Dursun; Salgado, Gilmar
Citation:

Citation: Korkut, Dursun Nizam; Alves, Isabel; Vogel, Alexander; Chabas, Sandrine; Sharma, Cynthia; Martinez, Denis; Loquet, Antoine; Salgado, Gilmar; Darfeuille, Fabien. "Structural insights into the AapA1 toxin of Helicobacter pylori"  Biochim. Biophys. Acta Gen. Subj. 1864, 129423-129423 (2020).
PubMed: 31476357

Assembly members:

Assembly members:
AapA1, polymer, 30 residues, 3435.22 Da.

Natural source:

Natural source:   Common Name: Helicobacter pylori   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AapA1: MATKHGKNSWKTLYLKISFL GCKVVVLLKR

Data sets:
Data typeCount
15N chemical shifts31
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AapA11

Entities:

Entity 1, AapA1 30 residues - 3435.22 Da.

1   METALATHRLYSHISGLYLYSASNSERTRP
2   LYSTHRLEUTYRLEULYSILESERPHELEU
3   GLYCYSLYSVALVALVALLEULEULYSARG

Samples:

sample_1: AapA1 1.5 ± 5 mM; AapA1 0.2 ± 5 mM; TFE 40%; acetic acid 10 mM; NaCl 10 mM; DTT 2 mM

sample_conditions_1: ionic strength: 30 mM; pH: 4.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TopSpin_3.1 v3.1, Bruker Biospin, CCPN, Goddard - chemical shift assignment, collection, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks