BMRB Entry 27461

Name: Human TGIF1 c-term domain
Published: 2018-07-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27461

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human TGIF1 c-term domain

Deposition date:

2018-04-23

Original release date:

2018-07-17

Authors:

Sunol Moreno, David; Macias, Maria J.

Citation:

Citation: Guca, Ewelina; Sunol, David; Ruiz, Lidia; Konkol, Agnieszka; Cordero, Jorge; Torner, Carles; Aragon, Eric; Martin-Malpartida, Pau; Riera, Antoni; Macias, Maria. "TGIF1 homeodomain interacts with Smad MH1 domain and represses TGF-beta signaling." Nucleic Acids Res. 46, 9220-9235 (2018).
PubMed: 30060237

Assembly members:

Assembly members:
TGIF1, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TGIF1: GAMGIKNFMPALEETPFHSC TAGPNPTLGRPLSPKPSSPG SVLARPSVICHTTVTALKDV PFSLCQSVGVGQNTDIQQIA AKNFTDTSLMYPED

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	149
15N chemical shifts	78
1H chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TGIF1	1

Entities:

Entity 1, TGIF1 94 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

ILE

LYS

ASN

PHE

MET

PRO

2

ALA

LEU

GLU

THR

PRO

PHE

HIS

SER

CYS

3

THR

ALA

GLY

PRO

ASN

PRO

THR

LEU

GLY

ARG

4

PRO

LEU

SER

PRO

LYS

PRO

SER

PRO

GLY

5

SER

VAL

LEU

ALA

ARG

PRO

SER

VAL

ILE

CYS

6

HIS

THR

VAL

THR

ALA

LEU

LYS

ASP

VAL

7

PRO

PHE

SER

LEU

CYS

GLN

SER

VAL

GLY

VAL

8

GLY

GLN

ASN

THR

ASP

ILE

GLN

ILE

ALA

9

ALA

LYS

ASN

PHE

THR

ASP

THR

SER

LEU

MET

10

TYR

PRO

GLU

ASP

Samples:

sample_1: TGIF1, [U-100% 13C; U-100% 15N], 100 mM; D2O, [U-2H], 10 % v/v; sodium phosphate 20 mM; sodium chloride 80 mM; TCEP 1 mM

sample_conditions_1: pH: 5.25; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

UNP	Q15583
AlphaFold	Q9NRS0