BMRB Entry 27457

Name: 13C, 15N solid-state NMR assignments of human histone H4 in nucleosome core particle
Published: 2018-07-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27457

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C, 15N solid-state NMR assignments of human histone H4 in nucleosome core particle

Deposition date:

2018-04-19

Original release date:

2018-07-30

Authors:

Shi, Xiangyan; Prasanna, Chinmayi; Pervushin, Konstantin; Nordenskiold, Lars

Citation:

Citation: Shi, Xiangyan; Prasanna, Chinmayi; Nagashima, Toshio; Yamazaki, Toshio; Pervushin, Konstantin; Nordenskiold, Lars. "Structure and Dynamics in the Nucleosome Revealed by Solid-State NMR" Angew. Chem. Int. Ed. Engl. 57, 9734-9738 (2018).
PubMed: 29905032

Assembly members:

Assembly members:
hH4-nucleosome, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hH4-nucleosome: SGRGKGGKGLGKGGAKRHRK VLRDNIQGITKPAIRRLARR GGVKRISGLIYEETRGVLKV FLENVIRDAVTYTEHAKRKT VTAMDVVYALKRQGRTLYGF GG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	294
15N chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hH4	1

Entities:

Entity 1, hH4 102 residues - Formula weight is not available

1

SER

GLY

ARG

GLY

LYS

GLY

LYS

GLY

LEU

2

GLY

LYS

GLY

ALA

LYS

ARG

HIS

ARG

LYS

3

VAL

LEU

ARG

ASP

ASN

ILE

GLN

GLY

ILE

THR

4

LYS

PRO

ALA

ILE

ARG

LEU

ALA

ARG

5

GLY

VAL

LYS

ARG

ILE

SER

GLY

LEU

ILE

6

TYR

GLU

THR

ARG

GLY

VAL

LEU

LYS

VAL

7

PHE

LEU

GLU

ASN

VAL

ILE

ARG

ASP

ALA

VAL

8

THR

TYR

THR

GLU

HIS

ALA

LYS

ARG

LYS

THR

9

VAL

THR

ALA

MET

ASP

VAL

TYR

ALA

LEU

10

LYS

ARG

GLN

GLY

ARG

THR

LEU

TYR

GLY

PHE

11

GLY

Samples:

sample_1: hH4-nucleosome, [U-100% 13C; U-100% 15N], 2.8 mg; hH2A-nucleosome 3.2 mg; hH2B-nucleosome 3.2 mg; hH3-nucleosome 3.5 mg; 601 Widom DNA 10.3 mg

sample_conditions_1: ionic strength: 20 mM; pH: 7.5; pressure: 1 atm; temperature: 285 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D CC DARR with 20 ms mixing time	sample_1	solid	sample_conditions_1
2D CC DARR with 100 ms mixing time	sample_1	solid	sample_conditions_1
2D NCA	sample_1	solid	sample_conditions_1
2D NCO	sample_1	solid	sample_conditions_1
3D NCACX with 100 ms CC DARR	sample_1	solid	sample_conditions_1
3D NCOCX with 100 ms CC DARR	sample_1	solid	sample_conditions_1
3D CANCO	sample_1	solid	sample_conditions_1

Software:

SPARKY v3.116, Goddard - chemical shift assignment

TOPSPIN v3.5pl6, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz