BMRB Entry 27449

Name: Backbone 1H, 13, 15N Chemical Shifts for Repressor of Primer (Rop) Variant C38A C52V (Ala-Val)
Published: 2018-09-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27449

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13, 15N Chemical Shifts for Repressor of Primer (Rop) Variant C38A C52V (Ala-Val)

Deposition date:

2018-04-12

Original release date:

2018-09-18

Authors:

Bowles, David; Yuan, Chunhua; Hansen, Alexandar; Stephany, Kimberly; Magliery, Thomas

Citation:

Citation: Bowles, David; Yuan, Chunhua; Stephany, Kimberly; Lavinder, Jason; Hansen, Alexandar; Magliery, Thomas. "Resonance assignments of wild-type and two cysteine-free variants of the four-helix bundle protein, Rop" Biomol. NMR Assignments 12, 345-350 (2018).
PubMed: 30159810

Assembly members:

Assembly members:
AV_Rop, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pmR-AVRop

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AV_Rop: GTKQEKTALNMARFIRSQTL TLLEKLNELDADEQADIAES LHDHADELYRSVLARFGDDG ENL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	123
15N chemical shifts	63
1H chemical shifts	63

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AV Rop monomer	1

Entities:

Entity 1, AV Rop monomer 63 residues - Formula weight is not available

1

GLY

THR

LYS

GLN

GLU

LYS

THR

ALA

LEU

ASN

2

MET

ALA

ARG

PHE

ILE

ARG

SER

GLN

THR

LEU

3

THR

LEU

GLU

LYS

LEU

ASN

GLU

LEU

ASP

4

ALA

ASP

GLU

GLN

ALA

ASP

ILE

ALA

GLU

SER

5

LEU

HIS

ASP

HIS

ALA

ASP

GLU

LEU

TYR

ARG

6

SER

VAL

LEU

ALA

ARG

PHE

GLY

ASP

GLY

7

GLU

ASN

LEU

Samples:

sample_1: AV Rop, [U-100% 13C; U-100% 15N], 1.35 mM; sodium phosphate 50 mM; sodium chloride 300 mM

sample_conditions_1: ionic strength: 1.2 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Ascend 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks