BMRB Entry 27446

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27446

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Title:
Assignment of the apo-form of the human C-terminal domain of UDP-Glucuronsyltransferase A1 (UGT1A)
Deposition date:
2018-04-10
Original release date:
2018-08-15
Authors:
Osborne, Michael; Coutinho de Oliveira, Luciana; Volpon, Laurent; Borden, Katherine
Citation:

Citation: Osborne, Michael; Coutinho de Oliveira, Luciana; Volpon, Laurent; Borden, Katherine. "Backbone assignment of the apo-form of the human C-terminal domain of UDP-glucuronosyltransferase 1A (UGT1A)"  Biomol. NMR Assignments 12, 315-318 (2018).
PubMed: 29934866

Assembly members:

Assembly members:
C-terminus_of_UGT1A4, polymer, 182 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-26b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminus_of_UGT1A4: MDIGINSDPGKPLSQEFEAY INASGEHGIVVFSLGSMVSE IPEKKAMAIADALGKIPQTV LWRYTGTRPSNLANNTILVK WLPQNDLLGHPMTRAFITHA GSHGVYESICNGVPMVMMPL FGDQMDNAKRMETKGAGVTL NVLEMTSEDLENALKAVIND KSYKENIMRLSSLHKDENLY FQ

Data sets:
Data typeCount
13C chemical shifts469
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ct of UGT1A4 monomer1

Entities:

Entity 1, Ct of UGT1A4 monomer 182 residues - Formula weight is not available

1   METASPILEGLYILEASNSERASPPROGLY
2   LYSPROLEUSERGLNGLUPHEGLUALATYR
3   ILEASNALASERGLYGLUHISGLYILEVAL
4   VALPHESERLEUGLYSERMETVALSERGLU
5   ILEPROGLULYSLYSALAMETALAILEALA
6   ASPALALEUGLYLYSILEPROGLNTHRVAL
7   LEUTRPARGTYRTHRGLYTHRARGPROSER
8   ASNLEUALAASNASNTHRILELEUVALLYS
9   TRPLEUPROGLNASNASPLEULEUGLYHIS
10   PROMETTHRARGALAPHEILETHRHISALA
11   GLYSERHISGLYVALTYRGLUSERILECYS
12   ASNGLYVALPROMETVALMETMETPROLEU
13   PHEGLYASPGLNMETASPASNALALYSARG
14   METGLUTHRLYSGLYALAGLYVALTHRLEU
15   ASNVALLEUGLUMETTHRSERGLUASPLEU
16   GLUASNALALEULYSALAVALILEASNASP
17   LYSSERTYRLYSGLUASNILEMETARGLEU
18   SERSERLEUHISLYSASPGLUASNLEUTYR
19   PHEGLN

Samples:

sample_1: C-terminus of UGT1A4, [U-100% 13C; U-100% 15N], 250 uM; sodium phosphate 50 mM; sodium chloride 300 mM; DTT 1 mM; sodium azide 0.02%

sample_2: C-terminus of UGT1A4, [U-13C; U-15N; U-2H], 250 uM; sodium phosphate 50 mM; sodium chloride 300 mM; DTT 1 mM; sodium azide 0.02%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

NCBI NP_009051

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks