Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27438
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Citation: Hilton, Jacob; Salehpour, Taraneh; Sisco, Nicholas; Rath, Parthasarathi; Van Horn, Wade. "Phosphoinositide-interacting regulator of TRP (PIRT) has opposing effects on human and mouse TRPM8 ion channels" J. Biol. Chem. 293, 9423-9434 (2018).
PubMed: 29724821
Assembly members:
Human_PIRT, polymer, 146 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b
Data type | Count |
13C chemical shifts | 141 |
15N chemical shifts | 91 |
1H chemical shifts | 92 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Human PIRT | 1 |
Entity 1, Human PIRT 146 residues - Formula weight is not available
1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | GLY | MET | ||||
2 | THR | MET | GLU | THR | LEU | PRO | LYS | VAL | LEU | GLU | ||||
3 | VAL | ASP | GLU | LYS | SER | PRO | GLU | ALA | LYS | ASP | ||||
4 | LEU | LEU | PRO | SER | GLN | THR | ALA | SER | SER | LEU | ||||
5 | CYS | ILE | SER | SER | ARG | SER | GLU | SER | VAL | TRP | ||||
6 | THR | THR | THR | PRO | ARG | SER | ASN | TRP | GLU | ILE | ||||
7 | TYR | ARG | LYS | PRO | ILE | VAL | ILE | MET | SER | VAL | ||||
8 | GLY | GLY | ALA | ILE | LEU | LEU | PHE | GLY | VAL | VAL | ||||
9 | ILE | THR | CYS | LEU | ALA | TYR | THR | LEU | LYS | LEU | ||||
10 | SER | ASP | LYS | SER | LEU | SER | ILE | LEU | LYS | MET | ||||
11 | VAL | GLY | PRO | GLY | PHE | LEU | SER | LEU | GLY | LEU | ||||
12 | MET | MET | LEU | VAL | CYS | GLY | LEU | VAL | TRP | VAL | ||||
13 | PRO | ILE | ILE | LYS | LYS | LYS | GLN | LYS | HIS | ARG | ||||
14 | GLN | LYS | SER | ASN | PHE | LEU | ARG | SER | LEU | LYS | ||||
15 | SER | PHE | PHE | LEU | THR | ARG |
sample_1: Human PIRT, [U-100% 13C; U-100% 15N], 500-750 uM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
CCPN, CCPN - chemical shift assignment, data analysis, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN, Bruker Biospin - collection
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks