BMRB Entry 27430

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27430

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Title:
Distinctive phosphoinositide and Ca2+ binding properties of normal and cognitive performance-linked variant forms of KIBRA C2 domain
Deposition date:
2018-03-19
Original release date:
2018-03-22
Authors:
Posner, Mareike; Upadhyay, Abhishek; Ishima, Rieko; Bagby, Stefan
Citation:

Citation: Posner, Mareike; Upadhyay, Abhishek; Ishima, Rieko; Kalli, Antreas; Harris, Gemma; Kremerskothen, Joachim; Sansom, Mark; Crennell, Susan; Bagby, Stefan. "Distinctive phosphoinositide- and Ca2+-binding properties of normal and cognitive performance-linked variant forms of KIBRA C2 domain"  J. Biol. Chem. 293, 9335-9344 (2018).
PubMed: 29724824

Assembly members:

Assembly members:
variant_KIBRA, polymer, 140 residues, 15809 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
variant_KIBRA: MRGSHHHHHHGSGATRIQIA LKYDEKNKQFAILIIQLSNL SALLQQQDQKVNIRVAVLPC SESTTCLFRTRPLDASDTLV FNEVFWVSIAYPALHQKTLR VDVCTTDRSHLEECLGGAQI SLAEVARSGERSTRWYNLLS

Data sets:
Data typeCount
13C chemical shifts245
15N chemical shifts116
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2 domain variant1

Entities:

Entity 1, C2 domain variant 140 residues - 15809 Da.

First 12 residues are a non-native affinity tag. Variant form (varmutC2) has IA instead of MS at positions 89 and 90.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERGLYALATHRARGILEGLNILEALA
3   LEULYSTYRASPGLULYSASNLYSGLNPHE
4   ALAILELEUILEILEGLNLEUSERASNLEU
5   SERALALEULEUGLNGLNGLNASPGLNLYS
6   VALASNILEARGVALALAVALLEUPROCYS
7   SERGLUSERTHRTHRCYSLEUPHEARGTHR
8   ARGPROLEUASPALASERASPTHRLEUVAL
9   PHEASNGLUVALPHETRPVALSERILEALA
10   TYRPROALALEUHISGLNLYSTHRLEUARG
11   VALASPVALCYSTHRTHRASPARGSERHIS
12   LEUGLUGLUCYSLEUGLYGLYALAGLNILE
13   SERLEUALAGLUVALALAARGSERGLYGLU
14   ARGSERTHRARGTRPTYRASNLEULEUSER

Samples:

sample_1: variant KIBRA, [U-98% 13C; U-98% 15N], 0.6 ± 0.1 mM; H2O 95 ± 1 %; D2O 5 ± 1 %; MES 50 ± 2 mM; sodium chloride 50 ± 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlysample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4, CCPN - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 700 MHz
  • Agilent Unity 800 MHz

Related Database Links:

NCBI NP_001155133

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks