BMRB Entry 27412

Name: dASCIZ LC8 binding domain (residues 241-388)
Published: 2018-03-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27412

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

dASCIZ LC8 binding domain (residues 241-388)

Deposition date:

2018-02-27

Original release date:

2018-03-02

Authors:

Clark, Sarah; Barbar, Elisar; Fiala, Radovan; Novacek, Jiri

Citation:

Citation: Clark, Sarah; Fiala, Radovan; Novacek, Jiri; Barbar, Elisar. "Assigned Chemical Shifts for dASCIZ LC8 Binding Domain (241-388)" .

Assembly members:

Assembly members:
dASCIZ, polymer, 148 residues, 17117.5 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET2Zt2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dASCIZ: MDVSYALEMSSQKLDMETQT EEDDLNEIRNEVLAPLLRDI ETQTPDTRGDIGTMTDDFPE EQEPVAVGSHFHAYSETEPM FDLQTSAHMYTQTCDDLFEE LGLSHIQTQTHWPDGLYNTQ HTQTCDEIMDELFPDNFQST CTQTRWLD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	362
15N chemical shifts	133
1H chemical shifts	468

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dASCIZ	1

Entities:

Entity 1, dASCIZ 148 residues - 17117.5 Da.

1

MET

ASP

VAL

SER

TYR

ALA

LEU

GLU

MET

SER

2

SER

GLN

LYS

LEU

ASP

MET

GLU

THR

GLN

THR

3

GLU

ASP

LEU

ASN

GLU

ILE

ARG

ASN

4

GLU

VAL

LEU

ALA

PRO

LEU

ARG

ASP

ILE

5

GLU

THR

GLN

THR

PRO

ASP

THR

ARG

GLY

ASP

6

ILE

GLY

THR

MET

THR

ASP

PHE

PRO

GLU

7

GLU

GLN

GLU

PRO

VAL

ALA

VAL

GLY

SER

HIS

8

PHE

HIS

ALA

TYR

SER

GLU

THR

GLU

PRO

MET

9

PHE

ASP

LEU

GLN

THR

SER

ALA

HIS

MET

TYR

10

THR

GLN

THR

CYS

ASP

LEU

PHE

GLU

11

LEU

GLY

LEU

SER

HIS

ILE

GLN

THR

GLN

THR

12

HIS

TRP

PRO

ASP

GLY

LEU

TYR

ASN

THR

GLN

13

HIS

THR

GLN

THR

CYS

ASP

GLU

ILE

MET

ASP

14

GLU

LEU

PHE

PRO

ASP

ASN

PHE

GLN

SER

THR

15

CYS

THR

GLN

THR

ARG

TRP

LEU

ASP

Samples:

sample_1: dASCIZ, [U-100% 13C; U-100% 15N], 0.3 mM; beta-mercaptoethanol 10 mM; sodium phosphate 10 mM; sodium chloride 10 mM; DSS 0.01 mM; sodium azide 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 283.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
5D HN(CA)CoNH	sample_1	isotropic	sample_conditions_1
5D HabCabCONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 850 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks