BMRB Entry 27402

Name: chemical shifts for human Axin DIX domain
Published: 2020-10-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27402

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

chemical shifts for human Axin DIX domain

Deposition date:

2018-02-14

Original release date:

2020-10-06

Authors:

Ward, Meaghan

Citation:

Citation: Ward, Meaghan; Daniels, Mark; van Kappel, Eline; Maurice, Madelon; Baldus, Marc. "Investigations of dynamic amyloid-like structures of the Wnt signalling pathway by solid-state NMR" Chem. Comm. 54, 3959-3962 (2018).
PubMed: 29561051

Assembly members:

Assembly members:
human_Axin_DIX_domain, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_Axin_DIX_domain: HHHHHENLYFQQPCDSIVVA YYFCGEPIPYRTLVRGRAVT LGQFKELLTKKGSYRYYFKK VSDEFDCGVVFEEVREDEAV LPVFEEKIIGKVEKVD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	273
15N chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Axin DIX domain, unit 1	1
2	Axin DIX domain, unit 2	1

Entities:

Entity 1, Axin DIX domain, unit 1 96 residues - Formula weight is not available

1

HIS

GLU

ASN

LEU

TYR

PHE

2

GLN

PRO

CYS

ASP

SER

ILE

VAL

ALA

3

TYR

PHE

CYS

GLY

GLU

PRO

ILE

PRO

TYR

4

ARG

THR

LEU

VAL

ARG

GLY

ARG

ALA

VAL

THR

5

LEU

GLY

GLN

PHE

LYS

GLU

LEU

THR

LYS

6

LYS

GLY

SER

TYR

ARG

TYR

PHE

LYS

7

VAL

SER

ASP

GLU

PHE

ASP

CYS

GLY

VAL

8

PHE

GLU

VAL

ARG

GLU

ASP

GLU

ALA

VAL

9

LEU

PRO

VAL

PHE

GLU

LYS

ILE

GLY

10

LYS

VAL

GLU

LYS

VAL

ASP

Samples:

sample_1: human Axin DIX domain, [U-100% 13C; U-100% 15N], solid ± 0 mM

sample_conditions_1: ionic strength: 20 mM; pH: 8; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D NCACX	sample_1	isotropic	sample_conditions_1
3D CANCO	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
2D CC Spin Diffusion for seqentials	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 950 MHz