BMRB Entry 27401

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27401

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Title:
Backbone assignment of Dynein Light Intermediate Chain 1 C-terminal domain (LIC1 Cterminal)
Deposition date:
2018-02-12
Original release date:
2019-01-02
Authors:
Henen, Morkos; Beat, Vogeli
Citation:

Citation: Henen, Morkos; Beat, Vogeli. "Dynein LIC1 C-terminal Domain backbone assignment. IDP by NMR"  Pos Biology ., .-..

Assembly members:

Assembly members:
LIC_C_terminal, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIC_C_terminal: GPWPTAAGRPVDASPRVPGG SPRTPNRSVSSNVASVSPIP AGSKKIDPNMKAGATSEGVL ANFFNSLLSKKTGSPGGPGV SGGSPAGGAGGGSSGLPPST KKSGQKPVLDVHAELDRITR KPVTVSPTTPTSPTEGEASG SGSGHHHHHH

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts105
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Single polypeptide IDP1

Entities:

Entity 1, Single polypeptide IDP 150 residues - Formula weight is not available

1   GLYPROTRPPROTHRALAALAGLYARGPRO
2   VALASPALASERPROARGVALPROGLYGLY
3   SERPROARGTHRPROASNARGSERVALSER
4   SERASNVALALASERVALSERPROILEPRO
5   ALAGLYSERLYSLYSILEASPPROASNMET
6   LYSALAGLYALATHRSERGLUGLYVALLEU
7   ALAASNPHEPHEASNSERLEULEUSERLYS
8   LYSTHRGLYSERPROGLYGLYPROGLYVAL
9   SERGLYGLYSERPROALAGLYGLYALAGLY
10   GLYGLYSERSERGLYLEUPROPROSERTHR
11   LYSLYSSERGLYGLNLYSPROVALLEUASP
12   VALHISALAGLULEUASPARGILETHRARG
13   LYSPROVALTHRVALSERPROTHRTHRPRO
14   THRSERPROTHRGLUGLYGLUALASERGLY
15   SERGLYSERGLYHISHISHISHISHISHIS

Samples:

sample_1: LIC C terminal, [U-100% 13C; U-100% 15N], 370 uM; D2O 5%; sodium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks