BMRB Entry 27395

Name: Backbone and methyl assignments for human Hsc70 substrate binding domain
Published: 2018-05-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27395

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and methyl assignments for human Hsc70 substrate binding domain

Deposition date:

2018-01-31

Original release date:

2018-05-09

Authors:

Zuiderweg, Erik; Morshauser, Robert

Citation:

Citation: Taylor, Isabelle; Ahmad, Atta; Wu, T.; Nordhues, Bryce; Bhullar, Anup; Gestwicki, Jason; Zuiderweg, Erik. "The disorderly conduct of Hsc70 and its interaction with the Alzheimer's related Tau protein" J. Biol. Chem. 293, 10796-10809 (2018).
PubMed: 29764935

Assembly members:

Assembly members:
Hsc70-SBD, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hsc70-SBD: MSNADVTPLSLGIETAGGVM TVLIKRNTTIPTKQTQTFTT YSDNQPGVLIQVYEGERAMT KDNNLLGKFELTGIPPAPRG VPQIEVTFDIDANGILNVSA VDKSTGKENKITITNDKGRL SKEDIERMVQEAEKYKAEDE KQQPGGGKVQIINKKLDGGG HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	460
15N chemical shifts	122
1H chemical shifts	362

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hsc70	1

Entities:

Entity 1, Hsc70 166 residues - Formula weight is not available

391-394 MSNA cloning artifact 395-532 HSC70 sequence 533-547 TAU sequence QPGGGKVQIINKKLDG 548-556 GGGHHHHHH HIS TAG

1

MET

SER

ASN

ALA

ASP

VAL

THR

PRO

LEU

SER

2

LEU

GLY

ILE

GLU

THR

ALA

GLY

VAL

MET

3

THR

VAL

LEU

ILE

LYS

ARG

ASN

THR

ILE

4

PRO

THR

LYS

GLN

THR

GLN

THR

PHE

THR

5

TYR

SER

ASP

ASN

GLN

PRO

GLY

VAL

LEU

ILE

6

GLN

VAL

TYR

GLU

GLY

GLU

ARG

ALA

MET

THR

7

LYS

ASP

ASN

LEU

GLY

LYS

PHE

GLU

8

LEU

THR

GLY

ILE

PRO

ALA

PRO

ARG

GLY

9

VAL

PRO

GLN

ILE

GLU

VAL

THR

PHE

ASP

ILE

10

ASP

ALA

ASN

GLY

ILE

LEU

ASN

VAL

SER

ALA

11

VAL

ASP

LYS

SER

THR

GLY

LYS

GLU

ASN

LYS

12

ILE

THR

ILE

THR

ASN

ASP

LYS

GLY

ARG

LEU

13

SER

LYS

GLU

ASP

ILE

GLU

ARG

MET

VAL

GLN

14

GLU

ALA

GLU

LYS

TYR

LYS

ALA

GLU

ASP

GLU

15

LYS

GLN

PRO

GLY

LYS

VAL

GLN

16

ILE

ASN

LYS

LEU

ASP

GLY

17

HIS

Samples:

HSC70-BETA-TAU: Hsc70 395-556, [U-100% 13C; U-100% 15N], 0.30 mM; Tris 25 mM; KCl 25 mM; EDTA 1 mM; DTT 1 mM; NaN3 0.02%

HSC70-BETA: Hsc70 395-508, [U-100% 13C; U-100% 15N], 0.5 mM; Ac-VQIINKKG-NH2 1 mM; Tris 25 mM; KCl 25 mM; EDTA 1 mM; DTT 1 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC TROSY	HSC70-BETA	isotropic	sample_conditions_1
3D HNCATROSY	HSC70-BETA	isotropic	sample_conditions_1
3D HN(CO)CATROSY	HSC70-BETA	isotropic	sample_conditions_1
3D HNCOTROSY	HSC70-BETA	isotropic	sample_conditions_1
3D HN(CA)COTROSY	HSC70-BETA	isotropic	sample_conditions_1
3D HNCACBTROSY	HSC70-BETA	isotropic	sample_conditions_1
2D 1H-15N HSQC	HSC70-BETA-TAU	isotropic	sample_conditions_1
2D 1H-15N HSQC TROSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D HNCATROSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D HN(CO)CATROSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D HNCOTROSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D HN(CA)COTROSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D HNCACBTROSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D 1H-15N NOESY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D HCCH-TOCSY	HSC70-BETA-TAU	isotropic	sample_conditions_1
3D 1H-13C NOESY	HSC70-BETA-TAU	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

SPARKY, Goddard - data analysis

EZ-ASSIGN v4.1, Zuiderweg - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks