BMRB Entry 27393

Name: 1H, 13C and 15N resonance assignments for a chemokine receptor-binding domain of FROUNT, a cytoplasmic regulator of chemotaxis
Published: 2018-04-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27393

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments for a chemokine receptor-binding domain of FROUNT, a cytoplasmic regulator of chemotaxis

Deposition date:

2018-01-30

Original release date:

2018-04-18

Authors:

Yoshinaga, Sosuke; Ishida, Norihito; Tsuji, Tatsuichiro; Sonoda, Akihiro; Yunoki, Kaori; Takeda, Mitsuhiro; Toda, Etsuko; Terashima, Yuya; Matsushima, Kouji; Terasawa, Hiroaki

Citation:

Citation: Yoshinaga, Sosuke; Ishida, Norihito; Tsuji, Tatsuichiro; Sonoda, Akihiro; Yunoki, Kaori; Takeda, Mitsuhiro; Toda, Etsuko; Terashima, Yuya; Matsushima, Kouji; Terasawa, Hiroaki. "1H, 13C and 15N resonance assignments for a chemokine receptor-binding domain of FROUNT, a cytoplasmic regulator of chemotaxis" Biomol. NMR Assignments 12, 259-262 (2018).
PubMed: 29594928

Assembly members:

Assembly members:
CRBD_LEPS, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CRBD_LEPS: GSDRLTFEGKYREFHRMYGE KRFADAASLLLSLMTSRIAP RSFWMTLLTDALPLLEQKQV IFSAEQTYELMRCLEDLTSR RSVHGESDTEQLQDDDIETT KVEMLRLSLARNLARAIIRE GSLEGS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	552
15N chemical shifts	127
1H chemical shifts	827

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CRBD_LEPS	1

Entities:

Entity 1, CRBD_LEPS 126 residues - Formula weight is not available

1

GLY

SER

ASP

ARG

LEU

THR

PHE

GLU

GLY

LYS

2

TYR

ARG

GLU

PHE

HIS

ARG

MET

TYR

GLY

GLU

3

LYS

ARG

PHE

ALA

ASP

ALA

SER

LEU

4

LEU

SER

LEU

MET

THR

SER

ARG

ILE

ALA

PRO

5

ARG

SER

PHE

TRP

MET

THR

LEU

THR

ASP

6

ALA

LEU

PRO

LEU

GLU

GLN

LYS

GLN

VAL

7

ILE

PHE

SER

ALA

GLU

GLN

THR

TYR

GLU

LEU

8

MET

ARG

CYS

LEU

GLU

ASP

LEU

THR

SER

ARG

9

ARG

SER

VAL

HIS

GLY

GLU

SER

ASP

THR

GLU

10

GLN

LEU

GLN

ASP

ILE

GLU

THR

11

LYS

VAL

GLU

MET

LEU

ARG

LEU

SER

LEU

ALA

12

ARG

ASN

LEU

ALA

ARG

ALA

ILE

ARG

GLU

13

GLY

SER

LEU

GLU

GLY

SER

Samples:

sample_1: CRBD_LEPS, [U-99% 13C; U-99% 15N], 0.2 mM; TRIS 20 mM; DTT 1 mM; D2O, [U-99% 2H], 5%

sample_2: CRBD_LEPS, [U-99% 13C; U-99% 15N], 0.2 mM; TRIS 20 mM; DTT 1 mM; D2O, [U-99% 2H], 100%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, The Olivia software - chemical shift assignment, peak picking, processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks