BMRB Entry 27376

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27376

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Title:
1H, 13C and 15N backbone resonance assignment of the prelamin A C-terminal region
Deposition date:
2018-01-22
Original release date:
2018-01-26
Authors:
Celli, Florian; Petitalot, Ambre; Samson, Camille; Theillet, Francois-Xavier; Zinn-Justin, Sophie
Citation:

Citation: Celli, Florian; Petitalot, Ambre; Samson, Camille; Theillet, Fran ois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region specific to prelamin A"  Biomol. NMR Assignments 12, 225-229 (2018).
PubMed: 29582385

Assembly members:

Assembly members:
Prelamin_A_CtoA, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Prelamin_A_CtoA: GMASHASSSGDPAEYNLRSR TVLAGTAGQPADKASASGSG AQVGGPISSGSSASSVTVTR SYRSVGGSGGGSFGDNLVTR SYLLGNSSPRTQSPQNASIM

Data sets:
Data typeCount
13C chemical shifts170
15N chemical shifts89
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Prelamin A C-terminal region1

Entities:

Entity 1, Prelamin A C-terminal region 100 residues - Formula weight is not available

1   GLYMETALASERHISALASERSERSERGLY
2   ASPPROALAGLUTYRASNLEUARGSERARG
3   THRVALLEUALAGLYTHRALAGLYGLNPRO
4   ALAASPLYSALASERALASERGLYSERGLY
5   ALAGLNVALGLYGLYPROILESERSERGLY
6   SERSERALASERSERVALTHRVALTHRARG
7   SERTYRARGSERVALGLYGLYSERGLYGLY
8   GLYSERPHEGLYASPASNLEUVALTHRARG
9   SERTYRLEULEUGLYASNSERSERPROARG
10   THRGLNSERPROGLNASNALASERILEMET

Samples:

sample_1: Prelamin A CtoA, [U-100% 13C; U-100% 15N], 600 uM; Phosphate Buffer, Natural Abundance, 20 mM; NaCl, Natural Abundance, 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Ponder - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks