BMRB Entry 27374

Name: 1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region of progerin
Published: 2018-01-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27374

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region of progerin

Deposition date:

2018-01-22

Original release date:

2018-01-26

Authors:

Zinn-Justin, Sophie

Citation:

Citation: Celli, Florian; Petitalot, Ambre; Samson, Camille; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the lamin C-terminal region specific to prelamin A" Biomol. NMR Assignments 12, 225-229 (2018).
PubMed: 29582385

Assembly members:

Assembly members:
Progerin_C-ter, polymer, 49 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Progerin_C-ter: WGSHCSSSGDPAEYNLRSRT VLCGTCGQPADKASASGSGA QSPQNCSIM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	128
15N chemical shifts	45
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Progerin C-ter	1

Entities:

Entity 1, Progerin C-ter 49 residues - Formula weight is not available

the first residue is an added tryptophane that is not present in the wild type peptide

1

TRP

GLY

SER

HIS

CYS

SER

GLY

ASP

2

PRO

ALA

GLU

TYR

ASN

LEU

ARG

SER

ARG

THR

3

VAL

LEU

CYS

GLY

THR

CYS

GLY

GLN

PRO

ALA

4

ASP

LYS

ALA

SER

ALA

SER

GLY

SER

GLY

ALA

5

GLN

SER

PRO

GLN

ASN

CYS

SER

ILE

MET

Samples:

sample_1: Progerin C-ter, [U-100% 13C; U-100% 15N], 600 uM; Phosphate Buffer, Natural Abundance, 20 mM; NaCl, Natural Abundance, 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

TINKER, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks