BMRB Entry 27355

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal P4 domain of AHNAK
Published: 2018-04-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27355

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal P4 domain of AHNAK

Deposition date:

2018-01-10

Original release date:

2018-04-18

Authors:

Shishmarev, Dmitry; Sundararaj, Srinivasan; Casarotto, Marco

Citation:

Citation: Sundararaj, Srinivasan; Shishmarev, Dmitry; Lin, Yiechang; Aditya, Shouvik; Casarotto, Marco. "1H, 13C and 15N backbone NMR chemical shift assignments of the C-terminal P4 domain of Ahnak" Biomol. NMR Assignments 12, 253-257 (2018).
PubMed: 29594929

Assembly members:

Assembly members:
AHNAK, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AHNAK: MGSSHHHHHHLEVLFQGPGS GGEVSLEGGKVKGKHGKLKF GTFGGLGSKSKGHYEVTGSD DETGKLQGSGVSLASKKSRL SSSSSNDSGNKVGIQLPEVE LSVSTKKE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	315
15N chemical shifts	94
1H chemical shifts	359

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AHNAK	1

Entities:

Entity 1, AHNAK 108 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

LEU

GLU

VAL

LEU

PHE

GLN

GLY

PRO

GLY

SER

3

GLY

GLU

VAL

SER

LEU

GLU

GLY

LYS

4

VAL

LYS

GLY

LYS

HIS

GLY

LYS

LEU

LYS

PHE

5

GLY

THR

PHE

GLY

LEU

GLY

SER

LYS

SER

6

LYS

GLY

HIS

TYR

GLU

VAL

THR

GLY

SER

ASP

7

ASP

GLU

THR

GLY

LYS

LEU

GLN

GLY

SER

GLY

8

VAL

SER

LEU

ALA

SER

LYS

SER

ARG

LEU

9

SER

ASN

ASP

SER

GLY

ASN

10

LYS

VAL

GLY

ILE

GLN

LEU

PRO

GLU

VAL

GLU

11

LEU

SER

VAL

SER

THR

LYS

GLU

Samples:

sample_1: AHNAK, [U-13C; U-15N], 0.5 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 150 mM; EDTA 2 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 15N TOCSY-HSQC	sample_1	isotropic	sample_conditions_1
3D 15N NOESY-HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

CCPNMR_Analysis v2.4.2, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks