BMRB Entry 27339

Name: Chemical shifts of UBQLN2 residues 450-624
Published: 2018-03-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27339

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of UBQLN2 residues 450-624

Deposition date:

2017-12-11

Original release date:

2018-03-16

Authors:

Castaneda, Carlos

Citation:

Citation: Dao, Thuy; Kolaitis, Regina-Maria; Kim, Hong Joo; O'Donovan, Kevin; Martyniak, Brian; Colicino, Erica; Hehnly, Heidi; Taylor, J Paul; Castaneda, Carlos. "Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions" Mol. Cell S1097, 30102-30103 (2018).
PubMed: 29526694

Assembly members:

Assembly members:
UBQLN2_450-624, polymer, 177 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UBQLN2_450-624: MRAMQALMQIQQGLQTLATE APGLIPSFTPGVGVGVLGTA IGPVGPVTPIGPIGPIVPFT PIGPIGPIGPTGPAAPPGST GSGGPTGPTVSSAAPSETTS PTSESGPNQQFIQQMVQALA GANAPQLPNPEVRFQQQLEQ LNAMGFLNREANLQALIATG GDINAAIERLLGSQPSW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	435
15N chemical shifts	147
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBQLN2	1

Entities:

Entity 1, UBQLN2 177 residues - Formula weight is not available

Second residue R is residue 450 of human UBQLN2. Additional C-terminal residue W for prot concentration measurements.

1

MET

ARG

ALA

MET

GLN

ALA

LEU

MET

GLN

ILE

2

GLN

GLY

LEU

GLN

THR

LEU

ALA

THR

GLU

3

ALA

PRO

GLY

LEU

ILE

PRO

SER

PHE

THR

PRO

4

GLY

VAL

GLY

VAL

GLY

VAL

LEU

GLY

THR

ALA

5

ILE

GLY

PRO

VAL

GLY

PRO

VAL

THR

PRO

ILE

6

GLY

PRO

ILE

GLY

PRO

ILE

VAL

PRO

PHE

THR

7

PRO

ILE

GLY

PRO

ILE

GLY

PRO

ILE

GLY

PRO

8

THR

GLY

PRO

ALA

PRO

GLY

SER

THR

9

GLY

SER

GLY

PRO

THR

GLY

PRO

THR

VAL

10

SER

ALA

PRO

SER

GLU

THR

SER

11

PRO

THR

SER

GLU

SER

GLY

PRO

ASN

GLN

12

PHE

ILE

GLN

MET

VAL

GLN

ALA

LEU

ALA

13

GLY

ALA

ASN

ALA

PRO

GLN

LEU

PRO

ASN

PRO

14

GLU

VAL

ARG

PHE

GLN

LEU

GLU

GLN

15

LEU

ASN

ALA

MET

GLY

PHE

LEU

ASN

ARG

GLU

16

ALA

ASN

LEU

GLN

ALA

LEU

ILE

ALA

THR

GLY

17

GLY

ASP

ILE

ASN

ALA

ILE

GLU

ARG

LEU

18

LEU

GLY

SER

GLN

PRO

SER

TRP

Samples:

13C_15N_450C: UBQLN2 450-624, [U-99% 13C; U-99% 15N], 200 uM; Na Phosphate 20 mM; EDTA 0.1 mM

buffer_conditions: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	13C_15N_450C	isotropic	buffer_conditions
3D HNCACB	13C_15N_450C	isotropic	buffer_conditions
3D CBCA(CO)NH	13C_15N_450C	isotropic	buffer_conditions
3D HNCA	13C_15N_450C	isotropic	buffer_conditions
3D HN(CA)CO	13C_15N_450C	isotropic	buffer_conditions
2D 1H-15N HSQC	13C_15N_450C	isotropic	buffer_conditions
2D N-CO (C-detect)	13C_15N_450C	isotropic	buffer_conditions
3D HACANCACON (C-detect)	13C_15N_450C	isotropic	buffer_conditions
3D HACANCANCO (C-detect)	13C_15N_450C	isotropic	buffer_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPNMR, CCPN - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks