BMRB Entry 27327

Name: Yes1 kinase SH3 domain
Published: 2018-01-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27327

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Yes1 kinase SH3 domain

Deposition date:

2017-12-03

Original release date:

2018-01-16

Authors:

Bachman, Ashleigh; Keramisanou, Dimitra; Kumar M.V., Vasantha; Gelis, Ioannis

Citation:

Citation: Bachman, Ashleigh; Keramisanou, Dimitra; Xu, Wanping; Beebe, Kristin; Moses, Michael; Vasantha Kumar, M; Gray, Geoffrey; Noor, Radwan Ebna; van der Vaart, Arjan; Neckers, Len; Gelis, Ioannis. "Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation" Nat. Commun. 9, 265-265 (2018).
PubMed: 29343704

Assembly members:

Assembly members:
Yes1-SH3, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PDB.HIS.GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yes1-SH3: GGVTIFVALYDYEARTTEDL SFKKGERFQIINNTEGDWWE ARSIATGKNGYIPSNYVAPA DSI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	117
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 63 residues - Formula weight is not available

1

GLY

VAL

THR

ILE

PHE

VAL

ALA

LEU

TYR

2

ASP

TYR

GLU

ALA

ARG

THR

GLU

ASP

LEU

3

SER

PHE

LYS

GLY

GLU

ARG

PHE

GLN

ILE

4

ILE

ASN

THR

GLU

GLY

ASP

TRP

GLU

5

ALA

ARG

SER

ILE

ALA

THR

GLY

LYS

ASN

GLY

6

TYR

ILE

PRO

SER

ASN

TYR

VAL

ALA

PRO

ALA

7

ASP

SER

ILE

Samples:

sample_1: Yes1-SH3, [U-100% 13C; U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Agilent direct drive 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks