BMRB Entry 27322
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27322
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NMR-STAR v3 text file.
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Citation: Bachman, Ashleigh; Keramisanou, Dimitra; Xu, Wanping; Beebe, Kristin; Moses, Michael; Vasantha Kumar, M; Gray, Geoffrey; Noor, Radwan Ebna; van der Vaart, Arjan; Neckers, Len; Gelis, Ioannis. "Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation" Nat. Commun. 9, 265-265 (2018).
PubMed: 29343704
Assembly members:
Cdc37_C-terminal_domain, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PDB.His.MBP
Entity Sequences (FASTA):
Cdc37_C-terminal_domain: GMGPGGLDPVEVEESLPEEL
QKCFDVKDVQMLQDAISKMD
PTDAKYHMQRCIDSGLWVPN
SKASEAKEGEEAGPGDPLLE
AVPKTGDEKDVSV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 226 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cdc37 | 1 |
Entities:
Entity 1, Cdc37 93 residues - Formula weight is not available
| 1 | GLY | MET | GLY | PRO | GLY | GLY | LEU | ASP | PRO | VAL | ||||
| 2 | GLU | VAL | GLU | GLU | SER | LEU | PRO | GLU | GLU | LEU | ||||
| 3 | GLN | LYS | CYS | PHE | ASP | VAL | LYS | ASP | VAL | GLN | ||||
| 4 | MET | LEU | GLN | ASP | ALA | ILE | SER | LYS | MET | ASP | ||||
| 5 | PRO | THR | ASP | ALA | LYS | TYR | HIS | MET | GLN | ARG | ||||
| 6 | CYS | ILE | ASP | SER | GLY | LEU | TRP | VAL | PRO | ASN | ||||
| 7 | SER | LYS | ALA | SER | GLU | ALA | LYS | GLU | GLY | GLU | ||||
| 8 | GLU | ALA | GLY | PRO | GLY | ASP | PRO | LEU | LEU | GLU | ||||
| 9 | ALA | VAL | PRO | LYS | THR | GLY | ASP | GLU | LYS | ASP | ||||
| 10 | VAL | SER | VAL |
Samples:
15N-13C: Cdc37 C-terminal domain, [U-100% 13C; U-100% 15N], 0.5 mM
methyl-labeled: Cdc37 C-terminal domain, methyl-labeled, 0.5 mM
15N: Cdc37, [U-100% 15N], 0.5 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | 15N-13C | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | 15N-13C | isotropic | sample_conditions_1 |
| 3D HNCACB | 15N-13C | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | 15N-13C | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | 15N | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | 15N | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | methyl-labeled | isotropic | sample_conditions_1 |
| CH3-CH3 HMQC-NOESY-HMQC | methyl-labeled | isotropic | sample_conditions_1 |
Software:
SPARKY, T. D. Goddard and D. G. Kneller - data analysis
NMR spectrometers:
- Agilent direct drive 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks