BMRB Entry 27321

Name: 1H, 13C and 15N Chemical Shift Assignments and 15N backbone relaxation data for intracellular loop 2 of the human ZIP4 protein
Published: 2019-06-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27321

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift Assignments and 15N backbone relaxation data for intracellular loop 2 of the human ZIP4 protein

Deposition date:

2017-11-30

Original release date:

2019-06-21

Authors:

Bafaro, Elizabeth; Maciejewski, Mark; Hoch, Jeffrey; Dempski, Robert

Citation:

Citation: Bafaro, Elizabeth; Maciejewski, Mark; Hoch, Jeffrey; Dempski, Robert. "Concomitant disorder and high-affinity zinc binding in the human zinc- and iron-regulated transport protein 4 intracellular loop" Protein Sci. 28, 868-880 (2019).
PubMed: 30793391

Assembly members:

Assembly members:
hZIP4_ICL2, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPRIBA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hZIP4_ICL2: GDRGPEFELGTLPRDPEDLE DGPCGHSSHSHGGHSHGVSL QLAPSELRQPKPPHEGSRAD LVAEESPELLNPEPRRLSPE LRLLPYGHGLSAWSHPQFEK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2
heteronucl_NOEs
- heteronuclear_noe_list_1
heteronucl_T1_relaxation
- heteronucl_T1_list_1
heteronucl_T2_relaxation
- heteronuclear_T2_list_1

Data type	Count
13C chemical shifts	350
15N chemical shifts	156
1H chemical shifts	385
T1 relaxation values	79
T2 relaxation values	79
heteronuclear NOE values	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hZIP4 ICL2	1

Entities:

Entity 1, hZIP4 ICL2 100 residues - Formula weight is not available

Residues 1-11 represent a cloning scar. Residues 87-100 represent a non-native affinity tag. Residues 12-86 are the intracellular loop of a membrane protein.

1	GLY	ASP	ARG	GLY	PRO	GLU	PHE	GLU	LEU	GLY
2	THR	LEU	PRO	ARG	ASP	PRO	GLU	ASP	LEU	GLU
3	ASP	GLY	PRO	CYS	GLY	HIS	SER	SER	HIS	SER
4	HIS	GLY	GLY	HIS	SER	HIS	GLY	VAL	SER	LEU
5	GLN	LEU	ALA	PRO	SER	GLU	LEU	ARG	GLN	PRO
6	LYS	PRO	PRO	HIS	GLU	GLY	SER	ARG	ALA	ASP
7	LEU	VAL	ALA	GLU	GLU	SER	PRO	GLU	LEU	LEU
8	ASN	PRO	GLU	PRO	ARG	ARG	LEU	SER	PRO	GLU
9	LEU	ARG	LEU	LEU	PRO	TYR	GLY	HIS	GLY	LEU
10	SER	ALA	TRP	SER	HIS	PRO	GLN	PHE	GLU	LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_2
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_2
T1_relaxation_800	sample_3	isotropic	sample_conditions_2
T2_relaxation_800	sample_3	isotropic	sample_conditions_2
HetNOE_relaxation_800	sample_3	isotropic	sample_conditions_2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 27321

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	ASP	ARG	GLY	PRO	GLU	PHE	GLU	LEU	GLY
2	THR	LEU	PRO	ARG	ASP	PRO	GLU	ASP	LEU	GLU
3	ASP	GLY	PRO	CYS	GLY	HIS	SER	SER	HIS	SER
4	HIS	GLY	GLY	HIS	SER	HIS	GLY	VAL	SER	LEU
5	GLN	LEU	ALA	PRO	SER	GLU	LEU	ARG	GLN	PRO
6	LYS	PRO	PRO	HIS	GLU	GLY	SER	ARG	ALA	ASP
7	LEU	VAL	ALA	GLU	GLU	SER	PRO	GLU	LEU	LEU
8	ASN	PRO	GLU	PRO	ARG	ARG	LEU	SER	PRO	GLU
9	LEU	ARG	LEU	LEU	PRO	TYR	GLY	HIS	GLY	LEU
10	SER	ALA	TRP	SER	HIS	PRO	GLN	PHE	GLU	LYS

1	GLY	ASP	ARG	GLY	PRO	GLU	PHE	GLU	LEU	GLY
2	THR	LEU	PRO	ARG	ASP	PRO	GLU	ASP	LEU	GLU
3	ASP	GLY	PRO	CYS	GLY	HIS	SER	SER	HIS	SER
4	HIS	GLY	GLY	HIS	SER	HIS	GLY	VAL	SER	LEU
5	GLN	LEU	ALA	PRO	SER	GLU	LEU	ARG	GLN	PRO
6	LYS	PRO	PRO	HIS	GLU	GLY	SER	ARG	ALA	ASP
7	LEU	VAL	ALA	GLU	GLU	SER	PRO	GLU	LEU	LEU
8	ASN	PRO	GLU	PRO	ARG	ARG	LEU	SER	PRO	GLU
9	LEU	ARG	LEU	LEU	PRO	TYR	GLY	HIS	GLY	LEU
10	SER	ALA	TRP	SER	HIS	PRO	GLN	PHE	GLU	LYS

1	GLY	ASP	ARG	GLY	PRO	GLU	PHE	GLU	LEU	GLY
2	THR	LEU	PRO	ARG	ASP	PRO	GLU	ASP	LEU	GLU
3	ASP	GLY	PRO	CYS	GLY	HIS	SER	SER	HIS	SER
4	HIS	GLY	GLY	HIS	SER	HIS	GLY	VAL	SER	LEU
5	GLN	LEU	ALA	PRO	SER	GLU	LEU	ARG	GLN	PRO
6	LYS	PRO	PRO	HIS	GLU	GLY	SER	ARG	ALA	ASP
7	LEU	VAL	ALA	GLU	GLU	SER	PRO	GLU	LEU	LEU
8	ASN	PRO	GLU	PRO	ARG	ARG	LEU	SER	PRO	GLU
9	LEU	ARG	LEU	LEU	PRO	TYR	GLY	HIS	GLY	LEU
10	SER	ALA	TRP	SER	HIS	PRO	GLN	PHE	GLU	LYS