BMRB Entry 27315

Name: Outer Membrane Protein X in PC14:1 MSPdH5 Nanodisc
Published: 2018-12-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27315

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Outer Membrane Protein X in PC14:1 MSPdH5 Nanodisc

Deposition date:

2017-11-22

Original release date:

2018-12-10

Authors:

Frey, Lukas; Riek, Roland; Bibow, Stefan

Citation:

Citation: Frey, Lukas; Hiller, Sebastian; Riek, Roland; Bibow, Stefan. "Lipid- and Cholesterol-Mediated Time-Scale-Specific Modulation of the Outer Membrane Protein X Dynamics in Lipid Bilayers" J. Am. Chem. Soc. 140, 15402-15411 (2018).
PubMed: 30289706

Assembly members:

Assembly members:
MSPdH5, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET_28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MSPdH5: ATSTVTGGYAQSDAQGQMNK MGGFNLKYRYEEDNSPLGVI GSFTYTEKSRTASSGDYNKN QYYGITAGPAYRINDWASIY GVVGVGYGKFQTTEYPTYKN DTSDYGFSYGAGLQFNPMEN VALDFSYEQSRIRSVDVGTW IAGVGYRF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	141
15N chemical shifts	144
1H chemical shifts	144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MSPdH5 Nanodisc	1

Entities:

Entity 1, MSPdH5 Nanodisc 148 residues - Formula weight is not available

1

ALA

THR

SER

THR

VAL

THR

GLY

TYR

ALA

2

GLN

SER

ASP

ALA

GLN

GLY

GLN

MET

ASN

LYS

3

MET

GLY

PHE

ASN

LEU

LYS

TYR

ARG

TYR

4

GLU

ASP

ASN

SER

PRO

LEU

GLY

VAL

ILE

5

GLY

SER

PHE

THR

TYR

THR

GLU

LYS

SER

ARG

6

THR

ALA

SER

GLY

ASP

TYR

ASN

LYS

ASN

7

GLN

TYR

GLY

ILE

THR

ALA

GLY

PRO

ALA

8

TYR

ARG

ILE

ASN

ASP

TRP

ALA

SER

ILE

TYR

9

GLY

VAL

GLY

VAL

GLY

TYR

GLY

LYS

PHE

10

GLN

THR

GLU

TYR

PRO

THR

TYR

LYS

ASN

11

ASP

THR

SER

ASP

TYR

GLY

PHE

SER

TYR

GLY

12

ALA

GLY

LEU

GLN

PHE

ASN

PRO

MET

GLU

ASN

13

VAL

ALA

LEU

ASP

PHE

SER

TYR

GLU

GLN

SER

14

ARG

ILE

ARG

SER

VAL

ASP

VAL

GLY

THR

TRP

15

ILE

ALA

GLY

VAL

GLY

TYR

ARG

PHE

Samples:

sample_1: OmpX in MSPdH5 Nanodisc, [U-100% 13C; U-100% 15N], 300 uM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 299.5 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Cambridge Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks