BMRB Entry 27312

Name: Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin
Published: 2018-03-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27312

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin

Deposition date:

2017-11-13

Original release date:

2018-03-29

Authors:

Bolton, David; Brown, Leonid; Ladizhansky, Vladimir

Citation:

Citation: Bolton, David; Brown, Leonid; Ladizhansky, Vladimir. "Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin" Biomol. NMR Assign. 12, 237-242 (2018).
PubMed: 29572785

Assembly members:

Assembly members:
ASR, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Anabaena variabilis Taxonomy ID: 264691 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena variabilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKJ606

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASR: MNLESLLHWIYVAGMTIGAL HFWSLSRNPRGVPQYEYLVA MFIPIWSGLAYMAMAIDQGK VEAAGQIAHYARYIDWMVTT PLLLLSLSWTAMQFIKKDWT LIGFLMSTQIVVITSGLIAD LSERDWVRYLWYICGVCAFL IILWGIWNPLRAKTRTQSSE LANLYDKLVTYFTVLWIGYP IVWIIGPSGFGWINQTIDTF LFCLLPFFSKVGFSFLDLHG LRNLNDSRQHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	186
15N chemical shifts	95
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ASR, chain 1	1
2	ASR, chain 2	1
3	ASR, chain 3	1

Entities:

Entity 1, ASR, chain 1 235 residues - Formula weight is not available

1

MET

ASN

LEU

GLU

SER

LEU

HIS

TRP

ILE

2

TYR

VAL

ALA

GLY

MET

THR

ILE

GLY

ALA

LEU

3

HIS

PHE

TRP

SER

LEU

SER

ARG

ASN

PRO

ARG

4

GLY

VAL

PRO

GLN

TYR

GLU

TYR

LEU

VAL

ALA

5

MET

PHE

ILE

PRO

ILE

TRP

SER

GLY

LEU

ALA

6

TYR

MET

ALA

MET

ALA

ILE

ASP

GLN

GLY

LYS

7

VAL

GLU

ALA

GLY

GLN

ILE

ALA

HIS

TYR

8

ALA

ARG

TYR

ILE

ASP

TRP

MET

VAL

THR

9

PRO

LEU

SER

LEU

SER

TRP

THR

10

ALA

MET

GLN

PHE

ILE

LYS

ASP

TRP

THR

11

LEU

ILE

GLY

PHE

LEU

MET

SER

THR

GLN

ILE

12

VAL

ILE

THR

SER

GLY

LEU

ILE

ALA

ASP

13

LEU

SER

GLU

ARG

ASP

TRP

VAL

ARG

TYR

LEU

14

TRP

TYR

ILE

CYS

GLY

VAL

CYS

ALA

PHE

LEU

15

ILE

LEU

TRP

GLY

ILE

TRP

ASN

PRO

LEU

16

ARG

ALA

LYS

THR

ARG

THR

GLN

SER

GLU

17

LEU

ALA

ASN

LEU

TYR

ASP

LYS

LEU

VAL

THR

18

TYR

PHE

THR

VAL

LEU

TRP

ILE

GLY

TYR

PRO

19

ILE

VAL

TRP

ILE

GLY

PRO

SER

GLY

PHE

20

GLY

TRP

ILE

ASN

GLN

THR

ILE

ASP

THR

PHE

21

LEU

PHE

CYS

LEU

PRO

PHE

SER

LYS

22

VAL

GLY

PHE

SER

PHE

LEU

ASP

LEU

HIS

GLY

23

LEU

ARG

ASN

LEU

ASN

ASP

SER

ARG

GLN

HIS

24

HIS

Samples:

UCND_ASR: Anabaena Sensory Rhodopsin, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mg

RAP_ASR: Anabaena Sensory Rhodopsin, [U-100% 13C; U-100% 15N, 15% 2H], 1 mg

sample_conditions_1: pH: 9; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CONH	UCND_ASR	isotropic	sample_conditions_1
3D CANH	UCND_ASR	isotropic	sample_conditions_1
3D (CO)CA(CO)NH	UCND_ASR	isotropic	sample_conditions_1
3D CO(CA)NH	UCND_ASR	isotropic	sample_conditions_1
3D CONH	RAP_ASR	isotropic	sample_conditions_1
3D CANH	RAP_ASR	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Keller and Wuthrich - data analysis, processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks