BMRB Entry 27301

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27301

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Title:
Backbone and side chain assignments of Scm3 from Saccharomyces cerevisiae
Deposition date:
2017-11-07
Original release date:
2019-04-12
Authors:
Bhattacharya, Anusri; Shukla, Vaibhav; Hosur, Ramakrishna; Kumar, Ashutosh
Citation:

Citation: Bhattacharya, Anusri; Shukla, Vaibhav; Hosur, Ramakrishna; Kumar, Ashutosh. "Backbone and side-chain resonance assignments of centromeric protein Scm3 from Saccharomyces cerevisiae"  Biomol. NMR Assign. 13, 267-273 (2019).
PubMed: 30937734

Assembly members:

Assembly members:
Scm3, polymer, 223 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHAT2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Scm3: MKTNKKISKRRSLKNLHGAL KGLLKESGKKSESKIRKHSD CNPVHRVYPPNIEKRKTKKD DGISRPIAERNGHVYIMSKE NHIIPKLTDDEVMERHKLAD ENMRKVWSNIISKYESIEEQ GDLVDLKTGEIVEDNGHIKT LTANNSTKDKRTKYTSVLRD IIDISDEEDGDKNDEYTLWA NDSEASDSEVDADNDTEEEK DEKLIDADFKKYEAKLSKRI LRD

Data sets:
Data typeCount
13C chemical shifts754
15N chemical shifts203
1H chemical shifts1247

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Scm31

Entities:

Entity 1, Scm3 223 residues - Formula weight is not available

1   METLYSTHRASNLYSLYSILESERLYSARG
2   ARGSERLEULYSASNLEUHISGLYALALEU
3   LYSGLYLEULEULYSGLUSERGLYLYSLYS
4   SERGLUSERLYSILEARGLYSHISSERASP
5   CYSASNPROVALHISARGVALTYRPROPRO
6   ASNILEGLULYSARGLYSTHRLYSLYSASP
7   ASPGLYILESERARGPROILEALAGLUARG
8   ASNGLYHISVALTYRILEMETSERLYSGLU
9   ASNHISILEILEPROLYSLEUTHRASPASP
10   GLUVALMETGLUARGHISLYSLEUALAASP
11   GLUASNMETARGLYSVALTRPSERASNILE
12   ILESERLYSTYRGLUSERILEGLUGLUGLN
13   GLYASPLEUVALASPLEULYSTHRGLYGLU
14   ILEVALGLUASPASNGLYHISILELYSTHR
15   LEUTHRALAASNASNSERTHRLYSASPLYS
16   ARGTHRLYSTYRTHRSERVALLEUARGASP
17   ILEILEASPILESERASPGLUGLUASPGLY
18   ASPLYSASNASPGLUTYRTHRLEUTRPALA
19   ASNASPSERGLUALASERASPSERGLUVAL
20   ASPALAASPASNASPTHRGLUGLUGLULYS
21   ASPGLULYSLEUILEASPALAASPPHELYS
22   LYSTYRGLUALALYSLEUSERLYSARGILE
23   LEUARGASP

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 500 mM; DTT 5 mM; Scm3, [U-15N], 1 mM

sample_2: sodium phosphate 20 mM; sodium chloride 500 mM; DTT 5 mM; Scm3, [U-13C; U-15N], 1 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCCcoNHsample_2isotropicsample_conditions_1
3D CCcoNHsample_2isotropicsample_conditions_1
3D HNNsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D MUSICsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker ASCEND 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks