BMRB Entry 27295

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27295

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Title:
Methyl-methionine resonance assignments for Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, Isoprenaline-bound form
Deposition date:
2017-10-26
Original release date:
2018-01-29
Authors:
Solt, Andras; Bostock, Mark; Shrestha, Binesh; Kumar, Prashant; Warne, Tony; Tate, Christopher; Nietlispach, Daniel
Citation:

Citation: Solt, Andras; Bostock, Mark; Shrestha, Binesh; Kumar, Prashant; Warne, Tony; Tate, Christopher; Nietlispach, Daniel. "Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound beta1-adrenergic receptor"  Nat. Commun. 8, 1795-1795 (2017).
PubMed: 29176642

Assembly members:

Assembly members:
Turkey_B1AR_D5_L190M, polymer, 317 residues, Formula weight is not available
entity_5FW, non-polymer, 211.258 Da.

Natural source:

Natural source:   Common Name: Turkey   Taxonomy ID: 9103   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meleagris gallopavo

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Spodoptera frugiperda   Vector: pBacPAK8

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Turkey_B1AR_D5_L190M: MGAELLSQQWEAGLSLLLAL VVLLIVAGNVLVIAAIGSTQ RLQTLTNLFITSLACADLVM GLLVVPFGATLVVRGTWLWG SFLCELWTSLDVLCVTASIW TLCVIAIDRYLAITSPFRYQ SLMTRARAKVIICTVWAISA LVSFLPIMLHWWRDEDPQAM KCYQDPGCCDFVTNRAYAIA SSIISFYIPLLIMIFVYLRV YREAKEQIRKIDRASKRKTS RVAAMREHKALKTLGIIMGV FTLCWLPFFLVNIVNVFNRD LVPDWLFVAFNWLGYANSAA NPIIYCRSPDFRKAFKRLLA FPRKADRRLHHHHHHHH

Data typeCount
13C chemical shifts14
1H chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 11
2Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 21
3Isoprenaline2

Entities:

Entity 1, Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 1 317 residues - Formula weight is not available

1   METGLYALAGLULEULEUSERGLNGLNTRP
2   GLUALAGLYLEUSERLEULEULEUALALEU
3   VALVALLEULEUILEVALALAGLYASNVAL
4   LEUVALILEALAALAILEGLYSERTHRGLN
5   ARGLEUGLNTHRLEUTHRASNLEUPHEILE
6   THRSERLEUALACYSALAASPLEUVALMET
7   GLYLEULEUVALVALPROPHEGLYALATHR
8   LEUVALVALARGGLYTHRTRPLEUTRPGLY
9   SERPHELEUCYSGLULEUTRPTHRSERLEU
10   ASPVALLEUCYSVALTHRALASERILETRP
11   THRLEUCYSVALILEALAILEASPARGTYR
12   LEUALAILETHRSERPROPHEARGTYRGLN
13   SERLEUMETTHRARGALAARGALALYSVAL
14   ILEILECYSTHRVALTRPALAILESERALA
15   LEUVALSERPHELEUPROILEMETLEUHIS
16   TRPTRPARGASPGLUASPPROGLNALAMET
17   LYSCYSTYRGLNASPPROGLYCYSCYSASP
18   PHEVALTHRASNARGALATYRALAILEALA
19   SERSERILEILESERPHETYRILEPROLEU
20   LEUILEMETILEPHEVALTYRLEUARGVAL
21   TYRARGGLUALALYSGLUGLNILEARGLYS
22   ILEASPARGALASERLYSARGLYSTHRSER
23   ARGVALALAALAMETARGGLUHISLYSALA
24   LEULYSTHRLEUGLYILEILEMETGLYVAL
25   PHETHRLEUCYSTRPLEUPROPHEPHELEU
26   VALASNILEVALASNVALPHEASNARGASP
27   LEUVALPROASPTRPLEUPHEVALALAPHE
28   ASNTRPLEUGLYTYRALAASNSERALAALA
29   ASNPROILEILETYRCYSARGSERPROASP
30   PHEARGLYSALAPHELYSARGLEULEUALA
31   PHEPROARGLYSALAASPARGARGLEUHIS
32   HISHISHISHISHISHISHIS

Entity 2, Isoprenaline - C11 H17 N O3 - 211.258 Da.

1   5FW

Samples:

L190M-Isoprenaline: Turkey_B1AR_D5_L190M, 13Ce-Met, 90 uM; Isoprenaline 1 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 8.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C SOFAST-HMQCL190M-Isoprenalineisotropicsample_conditions_1

Software:

CCPN_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz