BMRB Entry 27288

Name: RAP74-CTD
Published: 2017-12-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27288

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RAP74-CTD

Deposition date:

2017-10-20

Original release date:

2017-12-11

Authors:

De Mol, Eva; Bertoncini, Carlos; Fenwick, Bryn

Citation:

Citation: De Mol, Eva; Szulc, Elzbieta; Di Sanza, Claudio; Mart nez-Cristobal, Paula; Bertoncini, Carlos; Fenwick, Bryn; Frigol -Vivas, Marta; Masin, Marianela; Hunter, Irene; Buzon, Victor; Brun-Heath, Isabelle; Garcia, Jesus; De Fabritiis, Gianni; Estebanez-Perpi, Eva; McEwan, Iain; Nebreda, ngel; Salvatella, Xavier. "Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators" Structure 26, 145-152 (2018).
PubMed: 29225078

Assembly members:

Assembly members:
RAP74-CTD, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST-HisMBP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RAP74-CTD: GDVQVTEDAVRRYLTRKPMT TKDLLKKFQTKKTGLSSEQT VNVLAQILKRLNPERKMIND KMHFSLKE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	188
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RAP74-CTD, 1	1
2	RAP74-CTD, 2	1

Entities:

Entity 1, RAP74-CTD, 1 68 residues - Formula weight is not available

1

GLY

ASP

VAL

GLN

VAL

THR

GLU

ASP

ALA

VAL

2

ARG

TYR

LEU

THR

ARG

LYS

PRO

MET

THR

3

THR

LYS

ASP

LEU

LYS

PHE

GLN

THR

4

LYS

THR

GLY

LEU

SER

GLU

GLN

THR

5

VAL

ASN

VAL

LEU

ALA

GLN

ILE

LEU

LYS

ARG

6

LEU

ASN

PRO

GLU

ARG

LYS

MET

ILE

ASN

ASP

7

LYS

MET

HIS

PHE

SER

LEU

LYS

GLU

Samples:

sample_1: RAP74-CTD, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection, processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks