BMRB Entry 27270

Name: Backbone assignments of NS5A-D2 from Hepatitis C virus (JFH-1) phosphorylated by Casein Kinase II
Published: 2017-10-31

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27270

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of NS5A-D2 from Hepatitis C virus (JFH-1) phosphorylated by Casein Kinase II

Deposition date:

2017-10-03

Original release date:

2017-10-31

Authors:

Hanoulle, Xavier

Citation:

Citation: Bessa, Luiza; Schneider, Robert; Hanoulle, Xavier. "NMR and circular dichroism data for domain 2 of the HCV NS5A protein phosphorylated by the Casein Kinase II" Data Brief 17, .-325 (333).
PubMed: 29876401

Assembly members:

Assembly members:
pNS5A-D2, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Hepatitis C Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7.7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pNS5A-D2: MNTYDVDMVDANLLMEGGVA QTEPESRVPVLDFLEPMAEE EXDLEPSIPSECMLPRSGFP RALPAWARPDYNPPLVESWR RPDYQPPTVAGCALPLQHHH HHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	278
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pNS5A-D2	1

Entities:

Entity 1, pNS5A-D2 103 residues - Formula weight is not available

The first Methionine residue is a cloning artifact. The last 8 residues represent a non-native affinity tag. This is the domain 2 of the HCV (JFH1, genotype 2a) NS5A protein.

1

MET

ASN

THR

TYR

ASP

VAL

ASP

MET

VAL

ASP

2

ALA

ASN

LEU

MET

GLU

GLY

VAL

ALA

3

GLN

THR

GLU

PRO

GLU

SER

ARG

VAL

PRO

VAL

4

LEU

ASP

PHE

LEU

GLU

PRO

MET

ALA

GLU

5

GLU

SEP

ASP

LEU

GLU

PRO

SER

ILE

PRO

SER

6

GLU

CYS

MET

LEU

PRO

ARG

SER

GLY

PHE

PRO

7

ARG

ALA

LEU

PRO

ALA

TRP

ALA

ARG

PRO

ASP

8

TYR

ASN

PRO

LEU

VAL

GLU

SER

TRP

ARG

9

ARG

PRO

ASP

TYR

GLN

PRO

THR

VAL

ALA

10

GLY

CYS

ALA

LEU

PRO

LEU

GLN

HIS

11

HIS

Samples:

sample_1: pNS5A-D2, [U-100% 13C; U-100% 15N], 250 uM; sodium phosphate 20 mM; sodium chloride 30 mM; THP 1 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 0.03 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

In_house_product_plane_algorithm, in-house software - chemical shift calculation, data analysis

NMR spectrometers:

Bruker AvanceIII 800 MHz

Swiss-Prot	Q99IB8
GB	AB047639