BMRB Entry 27268
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27268
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NMR-STAR v3 text file.
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Citation: Halle-Bikovski, Adi; Fried, Sophia; Rozentur-Shkop, Eva; Biber, Guy; Shaked, Hadassa; Joseph, Noah; Baarda-Saad, Mira; Chill, Jordan. "New Structural Insights into Formation of the Key Actin Regulating WIP-WASp Complex Determined by NMR and Molecular Imaging" ACS Chem. Biol. 13, 100-109 (2018).
PubMed: 29215267
Assembly members:
WIP(442-492), polymer, 51 residues, Formula weight is not available
WASp(20-158), polymer, 139 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
WIP(442-492): QDSPCEDEWESRFYFHPISD
LPPPEPYVQTTKSYPSKLAR
NESRSGSNRRE
WASp(20-158): QNIPSTLLQDHENQRLFEML
GRKCLTLATAVVQLYLALPP
GAEHWTKEHCGAVCFVKDNP
QKSYFIRLYGLQAGRLLWEQ
ELYSQLVYSTPTPFFHTFAG
DDCQAGLNFADEDEAQAFRA
LVQEKIQKRNQRQSGDRRQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 526 |
| 15N chemical shifts | 167 |
| 1H chemical shifts | 563 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WIP | 1 |
| 2 | WASp | 2 |
Entities:
Entity 1, WIP 51 residues - Formula weight is not available
| 1 | GLN | ASP | SER | PRO | CYS | GLU | ASP | GLU | TRP | GLU | ||||
| 2 | SER | ARG | PHE | TYR | PHE | HIS | PRO | ILE | SER | ASP | ||||
| 3 | LEU | PRO | PRO | PRO | GLU | PRO | TYR | VAL | GLN | THR | ||||
| 4 | THR | LYS | SER | TYR | PRO | SER | LYS | LEU | ALA | ARG | ||||
| 5 | ASN | GLU | SER | ARG | SER | GLY | SER | ASN | ARG | ARG | ||||
| 6 | GLU |
Entity 2, WASp 139 residues - Formula weight is not available
| 1 | GLN | ASN | ILE | PRO | SER | THR | LEU | LEU | GLN | ASP | ||||
| 2 | HIS | GLU | ASN | GLN | ARG | LEU | PHE | GLU | MET | LEU | ||||
| 3 | GLY | ARG | LYS | CYS | LEU | THR | LEU | ALA | THR | ALA | ||||
| 4 | VAL | VAL | GLN | LEU | TYR | LEU | ALA | LEU | PRO | PRO | ||||
| 5 | GLY | ALA | GLU | HIS | TRP | THR | LYS | GLU | HIS | CYS | ||||
| 6 | GLY | ALA | VAL | CYS | PHE | VAL | LYS | ASP | ASN | PRO | ||||
| 7 | GLN | LYS | SER | TYR | PHE | ILE | ARG | LEU | TYR | GLY | ||||
| 8 | LEU | GLN | ALA | GLY | ARG | LEU | LEU | TRP | GLU | GLN | ||||
| 9 | GLU | LEU | TYR | SER | GLN | LEU | VAL | TYR | SER | THR | ||||
| 10 | PRO | THR | PRO | PHE | PHE | HIS | THR | PHE | ALA | GLY | ||||
| 11 | ASP | ASP | CYS | GLN | ALA | GLY | LEU | ASN | PHE | ALA | ||||
| 12 | ASP | GLU | ASP | GLU | ALA | GLN | ALA | PHE | ARG | ALA | ||||
| 13 | LEU | VAL | GLN | GLU | LYS | ILE | GLN | LYS | ARG | ASN | ||||
| 14 | GLN | ARG | GLN | SER | GLY | ASP | ARG | ARG | GLN |
Samples:
sample_1: WIP(442-492), [U-98% 13C; U-98% 15N], 0.5 mM; WASp(20-158), [U-98% 13C; U-98% 15N], 0.5 mM; D2O, [U-99% 2H], 7%; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 1 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - collection
NMR spectrometers:
- Bruker DRX 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks