BMRB Entry 27267

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27267

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for c-Jun
Deposition date:
2017-09-29
Original release date:
2017-10-31
Authors:
Csizmok, Veronika; Forman-Kay, Julie
Citation:

Citation: Csizmok, Veronika; Montecchio, Meri; Lin, Hong; Tyers, Mike; Sunnerhagen, Maria; Forman-Kay, Julie. "Multivalent Interactions with Fbw7 and Pin1 Facilitate Recognition of c-Jun by the SCFFbw7 Ubiquitin Ligase."  Structure 26, 28-39.e2 (2018).
PubMed: 29225075

Assembly members:

Assembly members:
JUN, polymer, 276 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-His-Sumo

Entity Sequences (FASTA):

Entity Sequences (FASTA):
JUN: MTAKMETTFYDDALNASFLP SESGPYGYSNPKILKQSMTL NLADPVGSLKPHLRAKNSDL LTSPDVGLLKLASPELERLI IQSSNGHITTTPTPTQFLCP KNVTDEQEGFAEGFVRALAE LHSQNTLPSVTSAAQPVNGA GMVAPAVASVAGGSGSGGFS ASLHSEPPVYANLSNFNPGA LSSGGGAPSYGAAGLAFPAQ PQQQQQPPHHLPQQMPVQHP RLQALKEEPQTVPEMPGETP PLSPIDMESQERIKAERKRM RNRIAASKCRKRKLER

Data sets:
Data typeCount
13C chemical shifts437
15N chemical shifts208
1H chemical shifts208

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Jun1

Entities:

Entity 1, Jun 276 residues - Formula weight is not available

1   METTHRALALYSMETGLUTHRTHRPHETYR
2   ASPASPALALEUASNALASERPHELEUPRO
3   SERGLUSERGLYPROTYRGLYTYRSERASN
4   PROLYSILELEULYSGLNSERMETTHRLEU
5   ASNLEUALAASPPROVALGLYSERLEULYS
6   PROHISLEUARGALALYSASNSERASPLEU
7   LEUTHRSERPROASPVALGLYLEULEULYS
8   LEUALASERPROGLULEUGLUARGLEUILE
9   ILEGLNSERSERASNGLYHISILETHRTHR
10   THRPROTHRPROTHRGLNPHELEUCYSPRO
11   LYSASNVALTHRASPGLUGLNGLUGLYPHE
12   ALAGLUGLYPHEVALARGALALEUALAGLU
13   LEUHISSERGLNASNTHRLEUPROSERVAL
14   THRSERALAALAGLNPROVALASNGLYALA
15   GLYMETVALALAPROALAVALALASERVAL
16   ALAGLYGLYSERGLYSERGLYGLYPHESER
17   ALASERLEUHISSERGLUPROPROVALTYR
18   ALAASNLEUSERASNPHEASNPROGLYALA
19   LEUSERSERGLYGLYGLYALAPROSERTYR
20   GLYALAALAGLYLEUALAPHEPROALAGLN
21   PROGLNGLNGLNGLNGLNPROPROHISHIS
22   LEUPROGLNGLNMETPROVALGLNHISPRO
23   ARGLEUGLNALALEULYSGLUGLUPROGLN
24   THRVALPROGLUMETPROGLYGLUTHRPRO
25   PROLEUSERPROILEASPMETGLUSERGLN
26   GLUARGILELYSALAGLUARGLYSARGMET
27   ARGASNARGILEALAALASERLYSCYSARG
28   LYSARGLYSLEUGLUARG

Samples:

sample_1: JUN, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 52 M; sodium phosphate 0.05 mM; DTT 0.02 mM; sodium chloride 0.15 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks