BMRB Entry 27258

Name: Hydrogen exchange rate of human galectin-8 CTD in apo and lactose-bound form
Published: 2017-11-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27258

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Hydrogen exchange rate of human galectin-8 CTD in apo and lactose-bound form

Deposition date:

2017-09-18

Original release date:

2017-11-17

Authors:

Chien, Chih-Ta Henry; Hsu, Shang-Te Danny

Citation:

Citation: Chien, Chih-Ta Henry; Ho, Meng-Ru; Lin, Chung-Hung; Hsu, Shang-Te Danny. "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange." Molecules 22, 1357-. (2017).
PubMed: 28813004

Assembly members:

Assembly members:
galectin-8_CTD, polymer, 135 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
galectin-8_CTD: MRLPFAARLNTPMGPGRTVV VKGEVNANAKSFNVDLLAGK SKDIALHLNPRLNIKAFVRN SFLQESWGEEERNITSFPFS PGMYFEMIIYCDVREFKVAV NGVHSLEYKHRFKELSSIDT LEINGDIHLLEVRSW

Data sets:

H_exch_rates
- H_exch_rate_list_1
- H_exch_rate_list_2

Data type	Count
H exchange rates	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	galectin-8 CTD in apo form	1

Entities:

Entity 1, galectin-8 CTD in apo form 135 residues - Formula weight is not available

1

MET

ARG

LEU

PRO

PHE

ALA

ARG

LEU

ASN

2

THR

PRO

MET

GLY

PRO

GLY

ARG

THR

VAL

3

VAL

LYS

GLY

GLU

VAL

ASN

ALA

ASN

ALA

LYS

4

SER

PHE

ASN

VAL

ASP

LEU

ALA

GLY

LYS

5

SER

LYS

ASP

ILE

ALA

LEU

HIS

LEU

ASN

PRO

6

ARG

LEU

ASN

ILE

LYS

ALA

PHE

VAL

ARG

ASN

7

SER

PHE

LEU

GLN

GLU

SER

TRP

GLY

GLU

8

GLU

ARG

ASN

ILE

THR

SER

PHE

PRO

PHE

SER

9

PRO

GLY

MET

TYR

PHE

GLU

MET

ILE

TYR

10

CYS

ASP

VAL

ARG

GLU

PHE

LYS

VAL

ALA

VAL

11

ASN

GLY

VAL

HIS

SER

LEU

GLU

TYR

LYS

HIS

12

ARG

PHE

LYS

GLU

LEU

SER

ILE

ASP

THR

13

LEU

GLU

ILE

ASN

GLY

ASP

ILE

HIS

LEU

14

GLU

VAL

ARG

SER

TRP

Samples:

sample_1: galectin-8 CTD, [U-100% 15N], 200 uM

sample_2: galectin-8 CTD, [U-100% 15N], 200 uM; Lactose 31.3 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 600 MHz