BMRB Entry 27257

Name: Hydrogen exchange rate of human galectin-8 NTD in apo and lactose-bound form
Published: 2017-11-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27257

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Hydrogen exchange rate of human galectin-8 NTD in apo and lactose-bound form

Deposition date:

2017-09-18

Original release date:

2017-11-17

Authors:

Chien, Chih-Ta Henry; Hsu, Shang-Te Danny

Citation:

Citation: Chien, Chih-Ta Henry; Ho, Meng-Ru; Lin, Chung-Hung; Hsu, Shang-Te Danny. "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange." Molecules 22, 1357-. (2017).
PubMed: 28813004

Assembly members:

Assembly members:
galectin-8_NTD, polymer, 155 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
galectin-8_NTD: MMLSLNNLQNIIYNPVIPFV GTIPDQLDPGTLIVIRGHVP SDADRFQVDLQNGSSMKPRA DVAFHFNPRFKRAGCIVCNT LINEKWGREEITYDTPFKRE KSFEIVIMVLKDKFQVAVNG KHTLLYGHRIGPEKIDTLGI YGKVNIHSIGFSFSS

Data sets:

H_exch_rates
- H_exch_rate_list_1
- H_exch_rate_list_2

Data type	Count
H exchange rates	138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	galectin-8 NTD in apo form	1

Entities:

Entity 1, galectin-8 NTD in apo form 155 residues - Formula weight is not available

1

MET

LEU

SER

LEU

ASN

LEU

GLN

ASN

2

ILE

TYR

ASN

PRO

VAL

ILE

PRO

PHE

VAL

3

GLY

THR

ILE

PRO

ASP

GLN

LEU

ASP

PRO

GLY

4

THR

LEU

ILE

VAL

ILE

ARG

GLY

HIS

VAL

PRO

5

SER

ASP

ALA

ASP

ARG

PHE

GLN

VAL

ASP

LEU

6

GLN

ASN

GLY

SER

MET

LYS

PRO

ARG

ALA

7

ASP

VAL

ALA

PHE

HIS

PHE

ASN

PRO

ARG

PHE

8

LYS

ARG

ALA

GLY

CYS

ILE

VAL

CYS

ASN

THR

9

LEU

ILE

ASN

GLU

LYS

TRP

GLY

ARG

GLU

10

ILE

THR

TYR

ASP

THR

PRO

PHE

LYS

ARG

GLU

11

LYS

SER

PHE

GLU

ILE

VAL

ILE

MET

VAL

LEU

12

LYS

ASP

LYS

PHE

GLN

VAL

ALA

VAL

ASN

GLY

13

LYS

HIS

THR

LEU

TYR

GLY

HIS

ARG

ILE

14

GLY

PRO

GLU

LYS

ILE

ASP

THR

LEU

GLY

ILE

15

TYR

GLY

LYS

VAL

ASN

ILE

HIS

SER

ILE

GLY

16

PHE

SER

PHE

SER

Samples:

sample_1: galectin-8 NTD, [U-100% 15N], 200 uM

sample_2: galectin-8 NTD, [U-100% 15N], 200 uM; Lactose 2.4 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 600 MHz