BMRB Entry 27256

Name: Hydrogen exchange rate of human galectin-7 in apo and lactose-bound form
Published: 2017-11-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27256

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Hydrogen exchange rate of human galectin-7 in apo and lactose-bound form

Deposition date:

2017-09-18

Original release date:

2017-11-17

Authors:

Chien, Chih-Ta Henry; Hsu, Shang-Te Danny

Citation:

Citation: Chien, Chih-Ta Henry; Ho, Meng-Ru; Lin, Chung-Hung; Hsu, Shang-Te Danny. "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange." Molecules 22, 1357-. (2017).
PubMed: 28813004

Assembly members:

Assembly members:
galectin-7, polymer, 135 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
galectin-7: SNVPHKSSLPEGIRPGTVLR IRGLVPPNASRFHVNLLCGE EQGSDAALHFNPRLDTSEVV FNSKEQGSWGREERGPGVPF QRGQPFEVLIIASDDGFKAV VGDAQYHHFRHRLPLARVRL VEVGGDVQLDSVRIF

Data sets:

H_exch_rates
- H_exch_rate_list_1
- H_exch_rate_list_2

Data type	Count
H exchange rates	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	galectin-7 in apo form	1

Entities:

Entity 1, galectin-7 in apo form 135 residues - Formula weight is not available

1

SER

ASN

VAL

PRO

HIS

LYS

SER

LEU

PRO

2

GLU

GLY

ILE

ARG

PRO

GLY

THR

VAL

LEU

ARG

3

ILE

ARG

GLY

LEU

VAL

PRO

ASN

ALA

SER

4

ARG

PHE

HIS

VAL

ASN

LEU

CYS

GLY

GLU

5

GLU

GLN

GLY

SER

ASP

ALA

LEU

HIS

PHE

6

ASN

PRO

ARG

LEU

ASP

THR

SER

GLU

VAL

7

PHE

ASN

SER

LYS

GLU

GLN

GLY

SER

TRP

GLY

8

ARG

GLU

ARG

GLY

PRO

GLY

VAL

PRO

PHE

9

GLN

ARG

GLY

GLN

PRO

PHE

GLU

VAL

LEU

ILE

10

ILE

ALA

SER

ASP

GLY

PHE

LYS

ALA

VAL

11

VAL

GLY

ASP

ALA

GLN

TYR

HIS

PHE

ARG

12

HIS

ARG

LEU

PRO

LEU

ALA

ARG

VAL

ARG

LEU

13

VAL

GLU

VAL

GLY

ASP

VAL

GLN

LEU

ASP

14

SER

VAL

ARG

ILE

PHE

Samples:

sample_1: galectin-7, [U-100% 15N], 200 uM

sample_2: galectin-7, [U-100% 15N], 200 uM; Lactose 6 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 600 MHz