BMRB Entry 27255

Name: Hydrogen exchange rate of human galectin-1 in apo and lactose-bound form
Published: 2017-11-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27255

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Hydrogen exchange rate of human galectin-1 in apo and lactose-bound form

Deposition date:

2017-09-18

Original release date:

2017-11-17

Authors:

Chien, Chih-Ta Henry; Hsu, Shang-Te Danny

Citation:

Citation: Chien, Chih-Ta Henry; Ho, Meng-Ru; Lin, Chung-Hung; Hsu, Shang-Te Danny. "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange." Molecules 22, 1357-. (2017).
PubMed: 28813004

Assembly members:

Assembly members:
galectin-1, polymer, 134 residues, Formula weight is not available
BETA-LACTOSE, non-polymer, 342.296 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
galectin-1: ASGLVASNLNLKPGECLRVR GEVAPDAKSFVLNLGKDSNN LCLHFNPRFNAHGDANTIVC NSKDDGAWGTEQREAVFPFQ PGSVAEVCITFDQANLTVKL PDGYEFKFPNRLNLEAINYM AADGDFKIKCVAFD

Data sets:

H_exch_rates
- H_exch_rate_list_1
- H_exch_rate_list_2

Data type	Count
H exchange rates	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	galectin-1 in apo form	1

Entities:

Entity 1, galectin-1 in apo form 134 residues - Formula weight is not available

1

ALA

SER

GLY

LEU

VAL

ALA

SER

ASN

LEU

ASN

2

LEU

LYS

PRO

GLY

GLU

CYS

LEU

ARG

VAL

ARG

3

GLY

GLU

VAL

ALA

PRO

ASP

ALA

LYS

SER

PHE

4

VAL

LEU

ASN

LEU

GLY

LYS

ASP

SER

ASN

5

LEU

CYS

LEU

HIS

PHE

ASN

PRO

ARG

PHE

ASN

6

ALA

HIS

GLY

ASP

ALA

ASN

THR

ILE

VAL

CYS

7

ASN

SER

LYS

ASP

GLY

ALA

TRP

GLY

THR

8

GLU

GLN

ARG

GLU

ALA

VAL

PHE

PRO

PHE

GLN

9

PRO

GLY

SER

VAL

ALA

GLU

VAL

CYS

ILE

THR

10

PHE

ASP

GLN

ALA

ASN

LEU

THR

VAL

LYS

LEU

11

PRO

ASP

GLY

TYR

GLU

PHE

LYS

PHE

PRO

ASN

12

ARG

LEU

ASN

LEU

GLU

ALA

ILE

ASN

TYR

MET

13

ALA

ASP

GLY

ASP

PHE

LYS

ILE

LYS

CYS

14

VAL

ALA

PHE

ASP

Samples:

sample_1: galectin-1, [U-100% 15N], 200 uM

sample_2: galectin-1, [U-100% 15N], 200 uM; Lactose 8.5 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 600 MHz