BMRB Entry 27252
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27252
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Citation: Bruschweiler, Sven; Yang, Qin; Run, Changqing; Chou, James. "Substrate Modulated Dynamics of the ADP/ATP Transporter Revealed by NMR Relaxation Dispersion" Nat. Struct. Biol. ., .-..
Assembly members:
yAAC3, polymer, 307 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
- chem_shift_perturbation
| Data type | Count |
| chemical shift perturbation values | 614 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yAAC3 | 1 |
Entities:
Entity 1, yAAC3 307 residues - Formula weight is not available
| 1 | ARG | GLY | SER | ASP | ALA | LYS | GLN | GLN | GLU | THR | ||||
| 2 | ASN | PHE | ALA | ILE | ASN | PHE | LEU | MET | GLY | GLY | ||||
| 3 | VAL | SER | ALA | ALA | ILE | ALA | LYS | THR | ALA | ALA | ||||
| 4 | SER | PRO | ILE | GLU | ARG | VAL | LYS | ILE | LEU | ILE | ||||
| 5 | GLN | ASN | GLN | ASP | GLU | MET | ILE | LYS | GLN | GLY | ||||
| 6 | THR | LEU | ASP | LYS | LYS | TYR | SER | GLY | ILE | VAL | ||||
| 7 | ASP | ALA | PHE | LYS | ARG | THR | ALA | LYS | GLN | GLU | ||||
| 8 | GLY | LEU | ILE | SER | PHE | TRP | ARG | GLY | ASN | THR | ||||
| 9 | ALA | ASN | VAL | ILE | ARG | TYR | PHE | PRO | THR | GLN | ||||
| 10 | ALA | LEU | ASN | PHE | ALA | PHE | LYS | ASP | LYS | ILE | ||||
| 11 | LYS | LEU | MET | PHE | GLY | PHE | LYS | LYS | GLU | GLU | ||||
| 12 | GLY | TYR | GLY | LYS | TRP | PHE | ALA | GLY | ASN | LEU | ||||
| 13 | ALA | SER | GLY | GLY | ALA | ALA | GLY | ALA | LEU | SER | ||||
| 14 | LEU | LEU | PHE | VAL | TYR | SER | LEU | ASP | PHE | ALA | ||||
| 15 | ARG | THR | ARG | LEU | ALA | ALA | ASP | ALA | LYS | SER | ||||
| 16 | SER | LYS | LYS | GLY | GLY | ALA | ARG | GLN | PHE | ASN | ||||
| 17 | GLY | LEU | THR | ASP | VAL | TYR | LYS | LYS | THR | LEU | ||||
| 18 | LYS | SER | ASP | GLY | ILE | ALA | GLY | LEU | TYR | ARG | ||||
| 19 | GLY | PHE | MET | PRO | SER | VAL | VAL | GLY | ILE | VAL | ||||
| 20 | VAL | TYR | ARG | GLY | LEU | TYR | PHE | GLY | MET | PHE | ||||
| 21 | ASP | SER | LEU | LYS | PRO | LEU | VAL | LEU | THR | GLY | ||||
| 22 | SER | LEU | ASP | GLY | SER | PHE | LEU | ALA | SER | PHE | ||||
| 23 | LEU | LEU | GLY | TRP | VAL | VAL | THR | THR | GLY | ALA | ||||
| 24 | SER | THR | VAL | SER | TYR | PRO | LEU | ASP | THR | VAL | ||||
| 25 | ARG | ARG | ARG | MET | MET | MET | THR | SER | GLY | GLN | ||||
| 26 | ALA | VAL | LYS | TYR | ASN | GLY | ALA | ILE | ASP | ALA | ||||
| 27 | LEU | LYS | LYS | ILE | VAL | ALA | SER | GLU | GLY | VAL | ||||
| 28 | GLY | SER | LEU | PHE | LYS | GLY | ALA | GLY | ALA | ASN | ||||
| 29 | ILE | LEU | ARG | SER | VAL | ALA | GLY | ALA | GLY | VAL | ||||
| 30 | ILE | SER | MET | TYR | ASP | GLN | LEU | GLN | MET | ILE | ||||
| 31 | LEU | PHE | GLY | LYS | LYS | PHE | LYS |
Samples:
sample_1: yAAC3, [U-100% 13C; U-100% 15N], 0.8 mM
sample_2: yAAC3, [U-100% 13C; U-100% 15N], 0.5 mM; CATR 3.5 mM
sample_3: yAAC3, [U-100% 15N], 0.5 mM; ADP 40 mM
sample_conditions_1: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 303.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CCPN, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz