BMRB Entry 27245

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27245

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Title:
Backbone 15N relaxation data for c-teminal domain of delta subunit of RNA polymerase from bacillus subtilis
Deposition date:
2017-09-06
Original release date:
2017-11-03
Authors:
Srb, Pavel; Novacek, Jiri; Kaderavek, Pavel; Rabatinova, Alzbeta; Krasny, Libor; Zidkova, Jitka; Bobalova, Jeanette; Sklenar, Vladimir; Zidek, Lukas
Citation:

Citation: Srb, Pavel; Novacek, Jiri; Kaderavek, Pavel; Rabatinova, Alzbeta; Krasny, Libor; Zidkova, Jitka; Bobalova, Jeanette; Sklenar, Vladimir; Zidek, Lukas. "Triple resonance [Formula: see text] NMR relaxation experiments for studies of intrinsically disordered proteins"  J. Biomol. NMR 69, 133-146 (2017).
PubMed: 29071460

Assembly members:

Assembly members:
delta, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
delta: MGIKQYSQEELKEMALVEIA HELFEEHKKPVPFQELLNEI ASLLGVKKEELGDRIAQFYT DLNIDGRFLALSDQTWGLRS WYPYDQLDEETQPTVKAKKK KAKKAVEEDLDLDEFEEIDE DDLDLDEVEEELDLEADDFD EEDLDEDDDDLEIEEDIIDE DDEDYDDEEEEIK

Data typeCount
T1 relaxation values285
T2 relaxation values144
dipole-dipole cross correlation relaxation values201
heteronuclear NOE values284

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1delta1

Entities:

Entity 1, delta 173 residues - Formula weight is not available

1   METGLYILELYSGLNTYRSERGLNGLUGLU
2   LEULYSGLUMETALALEUVALGLUILEALA
3   HISGLULEUPHEGLUGLUHISLYSLYSPRO
4   VALPROPHEGLNGLULEULEUASNGLUILE
5   ALASERLEULEUGLYVALLYSLYSGLUGLU
6   LEUGLYASPARGILEALAGLNPHETYRTHR
7   ASPLEUASNILEASPGLYARGPHELEUALA
8   LEUSERASPGLNTHRTRPGLYLEUARGSER
9   TRPTYRPROTYRASPGLNLEUASPGLUGLU
10   THRGLNPROTHRVALLYSALALYSLYSLYS
11   LYSALALYSLYSALAVALGLUGLUASPLEU
12   ASPLEUASPGLUPHEGLUGLUILEASPGLU
13   ASPASPLEUASPLEUASPGLUVALGLUGLU
14   GLULEUASPLEUGLUALAASPASPPHEASP
15   GLUGLUASPLEUASPGLUASPASPASPASP
16   LEUGLUILEGLUGLUASPILEILEASPGLU
17   ASPASPGLUASPTYRASPASPGLUGLUGLU
18   GLUILELYS

Samples:

sample_1: delta, [U-100% 13C; U-100% 15N], 0.8 mM; D2O, [U-2H], 10%; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; H2O 90%

sample_conditions_1: ionic strength: 30 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D NUS HNCO R1sample_1isotropicsample_conditions_1
3D NUS HNCO R2sample_1isotropicsample_conditions_1
3D NUS HNCO Heteronuclear NOEsample_1isotropicsample_conditions_1
3D NUS HNCO longitudinal cross-correlated cross-relaxationsample_1isotropicsample_conditions_1
3D NUS HNCO transverse cross-correlated cross-relaxationsample_1isotropicsample_conditions_1
3D NUS HNCO R1sample_1isotropicsample_conditions_1
3D NUS HNCO Heteronuclear NOEsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

SPARKY v3.115, Goddard - data analysis

cleaner3D, Jan Stanek, Viktor Kozminski - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz