BMRB Entry 27244
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27244
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Xie, Hua yong; Zhao, Yong xiang. "Chemical shifts assignments of Escherichia coli aquaporin (AqpZ) by solid-state NMR" Angew. Chem. Int. Ed. Engl. ., .-..
Assembly members:
AqpZ, polymer, 243 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSD005
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 840 |
| 15N chemical shifts | 220 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AqpZ | 1 |
Entities:
Entity 1, AqpZ 243 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLU | PHE | MET | PHE | ARG | LYS | LEU | ALA | ALA | GLU | ||||
| 3 | CYS | PHE | GLY | THR | PHE | TRP | LEU | VAL | PHE | GLY | ||||
| 4 | GLY | CYS | GLY | SER | ALA | VAL | LEU | ALA | ALA | GLY | ||||
| 5 | PHE | PRO | GLU | LEU | GLY | ILE | GLY | PHE | ALA | GLY | ||||
| 6 | VAL | ALA | LEU | ALA | PHE | GLY | LEU | THR | VAL | LEU | ||||
| 7 | THR | MET | ALA | PHE | ALA | VAL | GLY | HIS | ILE | SER | ||||
| 8 | GLY | GLY | HIS | PHE | ASN | PRO | ALA | VAL | THR | ILE | ||||
| 9 | GLY | LEU | TRP | ALA | GLY | GLY | ARG | PHE | PRO | ALA | ||||
| 10 | LYS | GLU | VAL | VAL | GLY | TYR | VAL | ILE | ALA | GLN | ||||
| 11 | VAL | VAL | GLY | GLY | ILE | VAL | ALA | ALA | ALA | LEU | ||||
| 12 | LEU | TYR | LEU | ILE | ALA | SER | GLY | LYS | THR | GLY | ||||
| 13 | PHE | ASP | ALA | ALA | ALA | SER | GLY | PHE | ALA | SER | ||||
| 14 | ASN | GLY | TYR | GLY | GLU | HIS | SER | PRO | GLY | GLY | ||||
| 15 | TYR | SER | MET | LEU | SER | ALA | LEU | VAL | VAL | GLU | ||||
| 16 | LEU | VAL | LEU | SER | ALA | GLY | PHE | LEU | LEU | VAL | ||||
| 17 | ILE | HIS | GLY | ALA | THR | ASP | LYS | PHE | ALA | PRO | ||||
| 18 | ALA | GLY | PHE | ALA | PRO | ILE | ALA | ILE | GLY | LEU | ||||
| 19 | ALA | LEU | THR | LEU | ILE | HIS | LEU | ILE | SER | ILE | ||||
| 20 | PRO | VAL | THR | ASN | THR | SER | VAL | ASN | PRO | ALA | ||||
| 21 | ARG | SER | THR | ALA | VAL | ALA | ILE | PHE | GLN | GLY | ||||
| 22 | GLY | TRP | ALA | LEU | GLU | GLN | LEU | TRP | PHE | PHE | ||||
| 23 | TRP | VAL | VAL | PRO | ILE | VAL | GLY | GLY | ILE | ILE | ||||
| 24 | GLY | GLY | LEU | ILE | TYR | ARG | THR | LEU | LEU | GLU | ||||
| 25 | LYS | ARG | ASP |
Samples:
sample_1: AqpZ, [U-13C; U-15N], 70 % w/w; POPC/POPG lipids % w/w
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D CONCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CANCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
VNMRJ v2.31A, Varian - collection
NMR spectrometers:
- Varian Uniform NMR System 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
| NCBI | GenBank: CCI77899.1 GenBank: CDO13762.1 |