BMRB Entry 27236

Name: Resonance assignment of the central conserved region (domains 8 to 14) of human tropoelastin
Published: 2017-09-27

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27236

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignment of the central conserved region (domains 8 to 14) of human tropoelastin

Deposition date:

2017-08-27

Original release date:

2017-09-27

Authors:

Reichheld, Sean; Sharpe, Simon

Citation:

Citation: Reichheld, Sean; Sharpe, Simon. "Resonance assignment and spectral density mapping of the central conserved region of tropoelastin" J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
hTE-CCR, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hTE-CCR: GAVVPQPGAGVKPGKVPGVG LPGVYPGGVLPGARFPGVGV LPGVPTGAGVKPKAPGVGGA FAGIPGVGPFGGPQPGVPLG YPIKAPKLPGGYGLPYTTGK LPYGYGPGGVAGAAGKAGYP TGT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	324
15N chemical shifts	118
1H chemical shifts	374

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hTE-CCR monomer	1

Entities:

Entity 1, hTE-CCR monomer 123 residues - Formula weight is not available

1

GLY

ALA

VAL

PRO

GLN

PRO

GLY

ALA

GLY

2

VAL

LYS

PRO

GLY

LYS

VAL

PRO

GLY

VAL

GLY

3

LEU

PRO

GLY

VAL

TYR

PRO

GLY

VAL

LEU

4

PRO

GLY

ALA

ARG

PHE

PRO

GLY

VAL

GLY

VAL

5

LEU

PRO

GLY

VAL

PRO

THR

GLY

ALA

GLY

VAL

6

LYS

PRO

LYS

ALA

PRO

GLY

VAL

GLY

ALA

7

PHE

ALA

GLY

ILE

PRO

GLY

VAL

GLY

PRO

PHE

8

GLY

PRO

GLN

PRO

GLY

VAL

PRO

LEU

GLY

9

TYR

PRO

ILE

LYS

ALA

PRO

LYS

LEU

PRO

GLY

10

GLY

TYR

GLY

LEU

PRO

TYR

THR

GLY

LYS

11

LEU

PRO

TYR

GLY

TYR

GLY

PRO

GLY

VAL

12

ALA

GLY

ALA

GLY

LYS

ALA

GLY

TYR

PRO

13

THR

GLY

THR

Samples:

sample_1: hTE-CCR, [U-13C; U-15N], 3.5 mM; potassium phosphate 50 mM; sodium chloride 350 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.35 M; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D NOESY-HSQC	sample_1	isotropic	sample_conditions_1
3D HSQC-TOCSY	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HN(CO)N	sample_1	isotropic	sample_conditions_1
3D HCAN	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks