BMRB Entry 27233

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for MazF from M. tuberculosis
Published: 2021-07-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27233

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for MazF from M. tuberculosis

Deposition date:

2017-08-24

Original release date:

2021-07-22

Authors:

Ahn, Do-Hwan

Citation:

Citation: Ahn, Do-Hwan; Lee, Ki-Young; Lee, Sang Jae; Park, Sung Jean; Yoon, Hye-Jin; Kim, Soon-Jong; Lee, Bong-Jin. "Structural analyses of the MazEF4 toxin-antitoxin pair in Mycobacterium tuberculosis provide evidence for a unique extracellular death factor" J. Biol. Chem. 292, 18832-18847 (2017).
PubMed: 28972145

Assembly members:

Assembly members:
MazF4, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MazF4: EFMNAPLRGQVYACDLGAGA KPWLIVSNNARNRHTADVVA VRLTTTRRTIPTWVAMGPSD PLTGYVNADNIETLGKDELG DYLGEVTPATMNKINTALAT ALGLPWP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	201
15N chemical shifts	76
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MazF4, Subunit 1	1
2	MazF4, Subunit 2	1

Entities:

Entity 1, MazF4, Subunit 1 107 residues - Formula weight is not available

1

GLU

PHE

MET

ASN

ALA

PRO

LEU

ARG

GLY

GLN

2

VAL

TYR

ALA

CYS

ASP

LEU

GLY

ALA

GLY

ALA

3

LYS

PRO

TRP

LEU

ILE

VAL

SER

ASN

ALA

4

ARG

ASN

ARG

HIS

THR

ALA

ASP

VAL

ALA

5

VAL

ARG

LEU

THR

ARG

THR

ILE

6

PRO

THR

TRP

VAL

ALA

MET

GLY

PRO

SER

ASP

7

PRO

LEU

THR

GLY

TYR

VAL

ASN

ALA

ASP

ASN

8

ILE

GLU

THR

LEU

GLY

LYS

ASP

GLU

LEU

GLY

9

ASP

TYR

LEU

GLY

GLU

VAL

THR

PRO

ALA

THR

10

MET

ASN

LYS

ILE

ASN

THR

ALA

LEU

ALA

THR

11

ALA

LEU

GLY

LEU

PRO

TRP

PRO

Samples:

sample_1: MazF4, [U-99% 13C; U-99% 15N], 0.3 mM; DTT 1 mM; NaCl 200 mM; MES 20 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D-HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Bruker Biospin - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks