BMRB Entry 27231

Name: Backbone assignments of the full length Dengue Virus NS4A protein in micelles
Published: 2017-11-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27231

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of the full length Dengue Virus NS4A protein in micelles

Deposition date:

2017-08-23

Original release date:

2017-11-03

Authors:

Li, Yan; Lee, Michelle Yueqi; Kang, CongBao

Citation:

Citation: Li, Yan; Lee, Michelle Yueqi; Loh, Ying Ru; Kang, CongBao. "Secondary structure and membrane topology of dengue virus NS4A protein in micelles" Biochim. Biophys. Acta S0005-2736, 30334-30336 (2017).
PubMed: 29055659

Assembly members:

Assembly members:
full_length_Dengue_NS4A, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Dengue Virus Taxonomy ID: 12637 Superkingdom: Viruses Kingdom: not available Genus/species: Dengue Virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
full_length_Dengue_NS4A: MSITLDILTEIASLPTYLSS RAKLALDNIVMLHTTERGGR AYQHALNELPESLETLMLVA LLGAMTAGIFLFFMQGKGIG KLSMGLITIAVASGLLWVAE IQPQWIAASIILEFFLMVLL IPEPEKQRLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	117
1H chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	full length Dengue NS4A	1

Entities:

Entity 1, full length Dengue NS4A 136 residues - Formula weight is not available

1

MET

SER

ILE

THR

LEU

ASP

ILE

LEU

THR

GLU

2

ILE

ALA

SER

LEU

PRO

THR

TYR

LEU

SER

3

ARG

ALA

LYS

LEU

ALA

LEU

ASP

ASN

ILE

VAL

4

MET

LEU

HIS

THR

GLU

ARG

GLY

ARG

5

ALA

TYR

GLN

HIS

ALA

LEU

ASN

GLU

LEU

PRO

6

GLU

SER

LEU

GLU

THR

LEU

MET

LEU

VAL

ALA

7

LEU

GLY

ALA

MET

THR

ALA

GLY

ILE

PHE

8

LEU

PHE

MET

GLN

GLY

LYS

GLY

ILE

GLY

9

LYS

LEU

SER

MET

GLY

LEU

ILE

THR

ILE

ALA

10

VAL

ALA

SER

GLY

LEU

TRP

VAL

ALA

GLU

11

ILE

GLN

PRO

GLN

TRP

ILE

ALA

SER

ILE

12

ILE

LEU

GLU

PHE

LEU

MET

VAL

LEU

13

ILE

PRO

GLU

PRO

GLU

LYS

GLN

ARG

LEU

GLU

14

HIS

Samples:

sample_1: full_length_Dengue_NS4A, [U-13C; U-15N; U-2H], 0.5 mM; sodium phosphate 20 mM; DPC 200 mM; DTT 1 mM; sodium chloride 70 mM

sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Keller and Wuthrich - chemical shift assignment, collection

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks