BMRB Entry 27229

Title:
hsa-miR-34a-mSIRT1 bulge U5C9mut
Deposition date:
2017-08-22
Original release date:
2020-05-21
Authors:
Baronti, Lorenzo; Steiner, Emilie; Schlagnitweit, Judith; Petzold, Katja
Citation:

Citation: Baronti, Lorenzo; Guzzetti, Ileana; Ebrahimi, Parisa; Friebe Sandoz, Sarah; Steiner, Emilie; Schlagnitweit, Judith; Fromm, Bastian; Silva, Luis; Fontana, Carolina; Chen, Alan; Petzold, Katja. "Base-pair Conformational Switch Modulates miR-34a Targeting of Sirt1 mRNA"  Nature 583, 139-144 (2020).
PubMed: 32461691

Assembly members:

Assembly members:
hsa-miR-34a-mSIRT1_bulge_U5C9mut, polymer, 28 residues, 8458.4 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis   Host organism: T7 in vitro transcritpion   Vector: DNA template

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hsa-miR-34a-mSIRT1_bulge_U5C9mut: GGCAUCAUCACUGCUUCGGC AGUGUGCC

Data sets:
Data typeCount
13C chemical shifts71
15N chemical shifts41
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hsa-miR-34a-mSIRT1 bulge U5C9mut1

Entities:

Entity 1, hsa-miR-34a-mSIRT1 bulge U5C9mut 28 residues - 8458.4 Da.

Nts 14-19 constitute a synthetic cUUCGg motif. Nts 1-3 and 26-28 synthetic closing stem. Nts 20-25 hsa-miR-34a-5p and nts 4-13 mSIRT1 side of the mutated hetero-duplex, respectively.

1   GGCAUCAUCA
2   CUGCUUCGGC
3   AGUGUGCC

Samples:

sample_1: hsa-miR-34a-mSIRT1 bulge U5C9mut, [U-100% 13C; U-100% 15N], 750 uM; H2O 90%; D2O, [U-2H], 10%; sodium phosphate 15 mM; sodium chloride 25 mM; EDTA 0.1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 295.4 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D HNN COSYsample_1isotropicsample_conditions_1
3D HCNsample_1isotropicsample_conditions_1

Software:

TOPSPIN vv 3.2, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III HD 600 MHz