BMRB Entry 27210

Name: PhoBN F20D assignment
Published: 2018-02-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27210

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

PhoBN F20D assignment

Deposition date:

2017-08-08

Original release date:

2018-02-02

Authors:

Kou, Xinhui

Citation:

Citation: Kou, Xinhui; Liu, Xinghong; Liu, Yixiang; Li, Conggang; Liu, Maili; Jiang, Ling. "Backbone resonance assignment of the Escherichia coli protein mutation PhoBNF20D" Biomol. NMR Assign. 12, 133-137 (2018).
PubMed: 29299752

Assembly members:

Assembly members:
PhoBNF20D, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PhoBNF20D: MARRILVVEDEAPIREMVCD VLEQNGFQPVEAEDYDSAVN QLNEPWPDLILLDWMLPGGS GIQFIKHLKRESMTRDIPVV MLTARGEEEDRVRGLETGAD DYITKPFSPKELVARIKAVM RRISQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	218
15N chemical shifts	109
1H chemical shifts	205

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PhoBNF20D	1

Entities:

Entity 1, PhoBNF20D 125 residues - Formula weight is not available

1

MET

ALA

ARG

ILE

LEU

VAL

GLU

ASP

2

GLU

ALA

PRO

ILE

ARG

GLU

MET

VAL

CYS

ASP

3

VAL

LEU

GLU

GLN

ASN

GLY

PHE

GLN

PRO

VAL

4

GLU

ALA

GLU

ASP

TYR

ASP

SER

ALA

VAL

ASN

5

GLN

LEU

ASN

GLU

PRO

TRP

PRO

ASP

LEU

ILE

6

LEU

ASP

TRP

MET

LEU

PRO

GLY

SER

7

GLY

ILE

GLN

PHE

ILE

LYS

HIS

LEU

LYS

ARG

8

GLU

SER

MET

THR

ARG

ASP

ILE

PRO

VAL

9

MET

LEU

THR

ALA

ARG

GLY

GLU

ASP

10

ARG

VAL

ARG

GLY

LEU

GLU

THR

GLY

ALA

ASP

11

ASP

TYR

ILE

THR

LYS

PRO

PHE

SER

PRO

LYS

12

GLU

LEU

VAL

ALA

ARG

ILE

LYS

ALA

VAL

MET

13

ARG

ILE

SER

GLN

Samples:

sample_1: PhoBNF20D, [U-98% 15N], 0.6 mM; PhoBNF20D, [U-95% 13C], 0.6 mM; soduim chloride 100 mM; DTT 10 mM; HEPES 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Uniform NMR System 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks