BMRB Entry 27209

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27209

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Title:
1HN, 13C, and 15N Resonance Assignments of the human DNA Ligase 3 DNA-Binding Domain (residues 257-477)
Deposition date:
2017-08-08
Original release date:
2019-07-10
Authors:
Roth, Braden; Varney, Kristen; Weber, David; Tomkinson, Alan
Citation:

Citation: Roth, Braden; Varney, Kristen; Yang, Hui; Weber, David; Tomkinson, Alan. "1HN, 13C, and 15N backbone resonance assignments of the human DNA ligase 3 DNA-binding domain (residues 257-477)"  Biomol. NMR Assignments 13, 305-308 (2019).
PubMed: 31093909

Assembly members:

Assembly members:
LIG3DBD, polymer, 222 residues, 25195.8182 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHGK-LIG3DBD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIG3DBD: SRHKDCLLREFRKLCAMVAD NPSYNTKTQIIQDFLRKGSA GDGFHGDVYLTVKLLLPGVI KTVYNLNDKQIVKLFSRIFN CNPDDMARDLEQGDVSETIR VFFEQSKSFPPAAKSLLTIQ EVDEFLLRLSKLTKEDEQQQ ALQDIASRCTANDLKCIIRL IKHDLKMNSGAKHVLDALDP NAYEAFKASRNLQDVVERVL HNAQEVEKEPGQRRALSVQA SL

Data sets:
Data typeCount
13C chemical shifts589
15N chemical shifts186
1H chemical shifts186

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LIG3DBD1

Entities:

Entity 1, LIG3DBD 222 residues - 25195.8182 Da.

residue 1 represents a non-native amino acid resulting from TEV cleavage of the protein from a fusion partner

1   SERARGHISLYSASPCYSLEULEUARGGLU
2   PHEARGLYSLEUCYSALAMETVALALAASP
3   ASNPROSERTYRASNTHRLYSTHRGLNILE
4   ILEGLNASPPHELEUARGLYSGLYSERALA
5   GLYASPGLYPHEHISGLYASPVALTYRLEU
6   THRVALLYSLEULEULEUPROGLYVALILE
7   LYSTHRVALTYRASNLEUASNASPLYSGLN
8   ILEVALLYSLEUPHESERARGILEPHEASN
9   CYSASNPROASPASPMETALAARGASPLEU
10   GLUGLNGLYASPVALSERGLUTHRILEARG
11   VALPHEPHEGLUGLNSERLYSSERPHEPRO
12   PROALAALALYSSERLEULEUTHRILEGLN
13   GLUVALASPGLUPHELEULEUARGLEUSER
14   LYSLEUTHRLYSGLUASPGLUGLNGLNGLN
15   ALALEUGLNASPILEALASERARGCYSTHR
16   ALAASNASPLEULYSCYSILEILEARGLEU
17   ILELYSHISASPLEULYSMETASNSERGLY
18   ALALYSHISVALLEUASPALALEUASPPRO
19   ASNALATYRGLUALAPHELYSALASERARG
20   ASNLEUGLNASPVALVALGLUARGVALLEU
21   HISASNALAGLNGLUVALGLULYSGLUPRO
22   GLYGLNARGARGALALEUSERVALGLNALA
23   SERLEU

Samples:

2H-13C-15N-LIG3DBD: LIG3DBD, [U-13C; U-15N; U-2H], 0.35 mM; MES 20 mM

15N-LIG3DBD: LIG3DBD, [U-13C; U-15N; U-2H], 0.69 mM; MES 20 mM

LIG3DBD_1: ionic strength: 0.008 M; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
3D HNCA2H-13C-15N-LIG3DBDisotropicLIG3DBD_1
3D HNCO2H-13C-15N-LIG3DBDisotropicLIG3DBD_1
3D HNCACB2H-13C-15N-LIG3DBDisotropicLIG3DBD_1
3D HN(CO)CA2H-13C-15N-LIG3DBDisotropicLIG3DBD_1
8 (HNcaCO)2H-13C-15N-LIG3DBDisotropicLIG3DBD_1
6 (H[N[co[{CA|ca[C]}]]])2H-13C-15N-LIG3DBDisotropicLIG3DBD_1
2D 1H-15N HSQC/HMQC15N-LIG3DBDisotropicLIG3DBD_1

Software:

CcpNmr_Analysis v2.4, CCPN - Analysis

NMR spectrometers:

  • Bruker AvanceIII 950 MHz

Related Database Links:

UniProt P49916
AlphaFold Q6NVK3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks