BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27208

Title: Fc fragment of mouse immunoglobulin G   PubMed: 28953258

Deposition date: 2017-08-07 Original release date: 2017-10-18

Authors: Yanaka, Saeko; Yamazaki, Toshio; Yogo, Rina; Yagi, Hirokazu; Kato, Koichi

Citation: Yanaka, Saeko; Yamazaki, Toshio; Yogo, Rina; Noda, Masanori; Uchiyama, Susumu; Yagi, Hirokazu; Kato, Koichi. "NMR Detection of Semi-Specific Antibody Interactions in Serum Environments."  Molecules 22, E1619-E1619 (2017).

Assembly members:
Fc_fragment_of_mouse_immunoglobulin_G, polymer, 219 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: mouse cell line   Vector: not applicable

Entity Sequences (FASTA):
Fc_fragment_of_mouse_immunoglobulin_G: CPAPNLEGGPSVFIFPPNIK DVLMISLTPKVTCVVVDVSE DDPDVQISWFVNNVEVHTAQ TQTHREDYNSTIRVVSTLPI QHQDWMSGKEFKCKVNNKDL PSPIERTISKIKGLVRAPQV YILPPPAEQLSRKDVSLTCL VVGFNPGDISVEWTSNGHTE ENYKDTAPVLDSDGSYFIYS KLNMKTSKWEKTDSFSCNVR HEGLKNYYLKKTISRSPGK

Data sets:
Data typeCount
13C chemical shifts425
15N chemical shifts197
1H chemical shifts197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fc fragment of mouse immunoglobulin G1

Entities:

Entity 1, Fc fragment of mouse immunoglobulin G 219 residues - Formula weight is not available

1   CYSPROALAPROASNLEUGLUGLYGLYPRO
2   SERVALPHEILEPHEPROPROASNILELYS
3   ASPVALLEUMETILESERLEUTHRPROLYS
4   VALTHRCYSVALVALVALASPVALSERGLU
5   ASPASPPROASPVALGLNILESERTRPPHE
6   VALASNASNVALGLUVALHISTHRALAGLN
7   THRGLNTHRHISARGGLUASPTYRASNSER
8   THRILEARGVALVALSERTHRLEUPROILE
9   GLNHISGLNASPTRPMETSERGLYLYSGLU
10   PHELYSCYSLYSVALASNASNLYSASPLEU
11   PROSERPROILEGLUARGTHRILESERLYS
12   ILELYSGLYLEUVALARGALAPROGLNVAL
13   TYRILELEUPROPROPROALAGLUGLNLEU
14   SERARGLYSASPVALSERLEUTHRCYSLEU
15   VALVALGLYPHEASNPROGLYASPILESER
16   VALGLUTRPTHRSERASNGLYHISTHRGLU
17   GLUASNTYRLYSASPTHRALAPROVALLEU
18   ASPSERASPGLYSERTYRPHEILETYRSER
19   LYSLEUASNMETLYSTHRSERLYSTRPGLU
20   LYSTHRASPSERPHESERCYSASNVALARG
21   HISGLUGLYLEULYSASNTYRTYRLEULYS
22   LYSTHRILESERARGSERPROGLYLYS

Samples:

sample_1: Fc fragment of mouse immunoglobulin G, [U-13C; U-15N], 10.4 mg/mL

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 325 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Bruker DRX 400 MHz
  • Bruker DMX 500 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts