BMRB Entry 27188

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27188

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Title:
N, H, CA, CB chemical shifts of E.coli CheA
Deposition date:
2017-07-21
Original release date:
2017-12-14
Authors:
Minato, Yuichi; Ueda, Takumi; Machiyama, Asako; Iwai, Hideo; Shimada, Ichio
Citation:

Citation: Minato, Yuichi; Ueda, Takumi; Machiyama, Asako; Iwai, Hideo; Shimada, Ichio. "Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR"  Sci. Rep. 7, 16462-16462 (2017).
PubMed: 29184123

Assembly members:

Assembly members:
CheA, polymer, 654 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET43a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CheA: MSMDISDFYQTFFDEADELL ADMEQHLLVLQPEAPDAEQL NAIFRAAHSIKGGAGTFGFS VLQETTHLMENLLDEARRGE MQLNTDIINLFLETKDIMQE QLDAYKQSQEPDAASFDYIC QALRQLALEAKGETPSAVTR LSVVAKSEPQDEQSRSQSPR RIILSRLKAGEVDLLEEELG HLTTLTDVVKGADSLSAILP GDIAEDDITAVLCFVIEADQ ITFETVEVSPKISTPPVLKL AAEQAPTGRVEREKTTRSNE STSIRVAVEKVDQLINLVGE LVITQSMLAQRSSELDPVNH GDLITSMGQLQRNARDLQES VMSIRMMPMEYVFSRYPRLV RDLAGKLGKQVELTLVGSST ELDKSLIERIIDPLTHLVRN SLDHGIELPEKRLAAGKNSV GNLILSAEHQGGNICIEVTD DGAGLNRERILAKAASQGLT VSENMSDDEVAMLIFAPGFS TAEQVTDVSGRGVGMDVVKR NIQEMGGHVEIQSKQGTGTT IRILLPLTLAILDGMSVRVA DEVFILPLNAVMESLQPREA DLHPLAGGERVLEVRGEYLP IVELWKVFNVAGAKTEATQG IVVILQSGGRRYALLVDQLI GQHQVVVKNLESNYRKVPGI SAATILGDGSVALIVDVSAL QAINREQRMANTAA

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts187
1H chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CheA1

Entities:

Entity 1, CheA 654 residues - Formula weight is not available

1   METSERMETASPILESERASPPHETYRGLN
2   THRPHEPHEASPGLUALAASPGLULEULEU
3   ALAASPMETGLUGLNHISLEULEUVALLEU
4   GLNPROGLUALAPROASPALAGLUGLNLEU
5   ASNALAILEPHEARGALAALAHISSERILE
6   LYSGLYGLYALAGLYTHRPHEGLYPHESER
7   VALLEUGLNGLUTHRTHRHISLEUMETGLU
8   ASNLEULEUASPGLUALAARGARGGLYGLU
9   METGLNLEUASNTHRASPILEILEASNLEU
10   PHELEUGLUTHRLYSASPILEMETGLNGLU
11   GLNLEUASPALATYRLYSGLNSERGLNGLU
12   PROASPALAALASERPHEASPTYRILECYS
13   GLNALALEUARGGLNLEUALALEUGLUALA
14   LYSGLYGLUTHRPROSERALAVALTHRARG
15   LEUSERVALVALALALYSSERGLUPROGLN
16   ASPGLUGLNSERARGSERGLNSERPROARG
17   ARGILEILELEUSERARGLEULYSALAGLY
18   GLUVALASPLEULEUGLUGLUGLULEUGLY
19   HISLEUTHRTHRLEUTHRASPVALVALLYS
20   GLYALAASPSERLEUSERALAILELEUPRO
21   GLYASPILEALAGLUASPASPILETHRALA
22   VALLEUCYSPHEVALILEGLUALAASPGLN
23   ILETHRPHEGLUTHRVALGLUVALSERPRO
24   LYSILESERTHRPROPROVALLEULYSLEU
25   ALAALAGLUGLNALAPROTHRGLYARGVAL
26   GLUARGGLULYSTHRTHRARGSERASNGLU
27   SERTHRSERILEARGVALALAVALGLULYS
28   VALASPGLNLEUILEASNLEUVALGLYGLU
29   LEUVALILETHRGLNSERMETLEUALAGLN
30   ARGSERSERGLULEUASPPROVALASNHIS
31   GLYASPLEUILETHRSERMETGLYGLNLEU
32   GLNARGASNALAARGASPLEUGLNGLUSER
33   VALMETSERILEARGMETMETPROMETGLU
34   TYRVALPHESERARGTYRPROARGLEUVAL
35   ARGASPLEUALAGLYLYSLEUGLYLYSGLN
36   VALGLULEUTHRLEUVALGLYSERSERTHR
37   GLULEUASPLYSSERLEUILEGLUARGILE
38   ILEASPPROLEUTHRHISLEUVALARGASN
39   SERLEUASPHISGLYILEGLULEUPROGLU
40   LYSARGLEUALAALAGLYLYSASNSERVAL
41   GLYASNLEUILELEUSERALAGLUHISGLN
42   GLYGLYASNILECYSILEGLUVALTHRASP
43   ASPGLYALAGLYLEUASNARGGLUARGILE
44   LEUALALYSALAALASERGLNGLYLEUTHR
45   VALSERGLUASNMETSERASPASPGLUVAL
46   ALAMETLEUILEPHEALAPROGLYPHESER
47   THRALAGLUGLNVALTHRASPVALSERGLY
48   ARGGLYVALGLYMETASPVALVALLYSARG
49   ASNILEGLNGLUMETGLYGLYHISVALGLU
50   ILEGLNSERLYSGLNGLYTHRGLYTHRTHR
51   ILEARGILELEULEUPROLEUTHRLEUALA
52   ILELEUASPGLYMETSERVALARGVALALA
53   ASPGLUVALPHEILELEUPROLEUASNALA
54   VALMETGLUSERLEUGLNPROARGGLUALA
55   ASPLEUHISPROLEUALAGLYGLYGLUARG
56   VALLEUGLUVALARGGLYGLUTYRLEUPRO
57   ILEVALGLULEUTRPLYSVALPHEASNVAL
58   ALAGLYALALYSTHRGLUALATHRGLNGLY
59   ILEVALVALILELEUGLNSERGLYGLYARG
60   ARGTYRALALEULEUVALASPGLNLEUILE
61   GLYGLNHISGLNVALVALVALLYSASNLEU
62   GLUSERASNTYRARGLYSVALPROGLYILE
63   SERALAALATHRILELEUGLYASPGLYSER
64   VALALALEUILEVALASPVALSERALALEU
65   GLNALAILEASNARGGLUGLNARGMETALA
66   ASNTHRALAALA

Samples:

sample_1: CheA, [U-13C; U-15N; U-2H], 0.3 mM; TRIS 50 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks