BMRB Entry 27187
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27187
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Citation: Corbeski, Ivan; Dolinar, Klemen; Wienk, Hans; Boelens, Rolf; van Ingen, Hugo. "DNA repair factor APLF acts as a H2A-H2B histone chaperone through binding its DNA interaction surface" Nucleic Acids Res. 46, 7138-7152 (2018).
PubMed: 29905837
Assembly members:
H2B_in_the_H2A-H2B_complex, polymer, 123 residues, Formula weight is not available
H2A, polymer, 123 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b
Entity Sequences (FASTA):
H2B_in_the_H2A-H2B_complex: IPPKTSGKAAKKAGKAQKNI
TKTDKKKKRKRKESYAIYIY
KVLKQVHPDTGISSKAMSIM
NSFVNDIFERIAAEASRLAH
YNKRSTITSREIQTAVRLLL
PGELAKHAVSEGTKAVTKYT
SSK
H2A: SGRGKGGKVKGKAKSRSNRA
GLQFPVGRIHRLLRKGNYAE
RVGAGAPVYLAAVMEYLAAE
VLELAGNAARDNKKTRIIPR
HLQLAIRNDEELNKLLSGVT
IAQGGVLPNIQAVLLPKKTE
KKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 342 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 112 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H2B | 1 |
| 2 | H2A | 2 |
Entities:
Entity 1, H2B 123 residues - Formula weight is not available
| 1 | ILE | PRO | PRO | LYS | THR | SER | GLY | LYS | ALA | ALA | ||||
| 2 | LYS | LYS | ALA | GLY | LYS | ALA | GLN | LYS | ASN | ILE | ||||
| 3 | THR | LYS | THR | ASP | LYS | LYS | LYS | LYS | ARG | LYS | ||||
| 4 | ARG | LYS | GLU | SER | TYR | ALA | ILE | TYR | ILE | TYR | ||||
| 5 | LYS | VAL | LEU | LYS | GLN | VAL | HIS | PRO | ASP | THR | ||||
| 6 | GLY | ILE | SER | SER | LYS | ALA | MET | SER | ILE | MET | ||||
| 7 | ASN | SER | PHE | VAL | ASN | ASP | ILE | PHE | GLU | ARG | ||||
| 8 | ILE | ALA | ALA | GLU | ALA | SER | ARG | LEU | ALA | HIS | ||||
| 9 | TYR | ASN | LYS | ARG | SER | THR | ILE | THR | SER | ARG | ||||
| 10 | GLU | ILE | GLN | THR | ALA | VAL | ARG | LEU | LEU | LEU | ||||
| 11 | PRO | GLY | GLU | LEU | ALA | LYS | HIS | ALA | VAL | SER | ||||
| 12 | GLU | GLY | THR | LYS | ALA | VAL | THR | LYS | TYR | THR | ||||
| 13 | SER | SER | LYS |
Entity 2, H2A 123 residues - Formula weight is not available
| 1 | SER | GLY | ARG | GLY | LYS | GLY | GLY | LYS | VAL | LYS | ||||
| 2 | GLY | LYS | ALA | LYS | SER | ARG | SER | ASN | ARG | ALA | ||||
| 3 | GLY | LEU | GLN | PHE | PRO | VAL | GLY | ARG | ILE | HIS | ||||
| 4 | ARG | LEU | LEU | ARG | LYS | GLY | ASN | TYR | ALA | GLU | ||||
| 5 | ARG | VAL | GLY | ALA | GLY | ALA | PRO | VAL | TYR | LEU | ||||
| 6 | ALA | ALA | VAL | MET | GLU | TYR | LEU | ALA | ALA | GLU | ||||
| 7 | VAL | LEU | GLU | LEU | ALA | GLY | ASN | ALA | ALA | ARG | ||||
| 8 | ASP | ASN | LYS | LYS | THR | ARG | ILE | ILE | PRO | ARG | ||||
| 9 | HIS | LEU | GLN | LEU | ALA | ILE | ARG | ASN | ASP | GLU | ||||
| 10 | GLU | LEU | ASN | LYS | LEU | LEU | SER | GLY | VAL | THR | ||||
| 11 | ILE | ALA | GLN | GLY | GLY | VAL | LEU | PRO | ASN | ILE | ||||
| 12 | GLN | ALA | VAL | LEU | LEU | PRO | LYS | LYS | THR | GLU | ||||
| 13 | LYS | LYS | ALA |
Samples:
sample_1: H2B in the H2A-H2B complex, [U-100% 13C; U-100% 15N; U-100% 2H], 500 uM; potassium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02 % w/v
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks