BMRB Entry 27183

Name: 1H, 15N, 13C backbone resonance assignment of the C-terminal domain of Enzyme I from Thermoanaerobacter Tengcongensis
Published: 2017-11-07

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27183

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C backbone resonance assignment of the C-terminal domain of Enzyme I from Thermoanaerobacter Tengcongensis

Deposition date:

2017-07-18

Original release date:

2017-11-07

Authors:

Dotas, Rochelle; Venditti, Vincenzo

Citation:

Citation: Dotas, Rochelle; Venditti, Vincenzo. "1H, 15N, 13C backbone resonance assignment of the C-terminal domain of enzyme I from Thermoanaerobacter tengcongensis" Biomol. NMR Assignments 12, 103-106 (2018).
PubMed: 29064000

Assembly members:

Assembly members:
EIC_from_from_Thermoanaerobacter_Tengcongensis, polymer, 314 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thermoanaerobacter tengcongensis Taxonomy ID: 119072 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermoanaerobacter tengcongensis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EIC_from_from_Thermoanaerobacter_Tengcongensis: MAETPDGKKVMLAANIGTPK DVASALANGAEGVGLFRTEF LYMDRNSLPSEEEQFEAYKE VVEKMGGRPVTIRTLDIGGD KELPYLDMPKEMNPFLGYRA IRLCLDRPDIFKTQLRAILR ASAYGNVQIMYPMISSVEEV RKANSILEEVKAELDREGVK YDKEIKVGIMVEIPSAAVTA DILAKEVDFFSIGTNDLTQY TLAVDRMNEHVKEYYQPFHP AILRLVKMVIDAAHKEGKFA AMCGEMAGDPLAAVILLGLG LDEFSMSATSIPEIKNIIRN VEYEKAKEIAEKALNMSEAR EIEKMMKDVIKDIG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	821
15N chemical shifts	264
1H chemical shifts	264

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EIC subunit 1	1
2	EIC subunit 2	1

Entities:

Entity 1, EIC subunit 1 314 residues - Formula weight is not available

1

MET

ALA

GLU

THR

PRO

ASP

GLY

LYS

VAL

2

MET

LEU

ALA

ASN

ILE

GLY

THR

PRO

LYS

3

ASP

VAL

ALA

SER

ALA

LEU

ALA

ASN

GLY

ALA

4

GLU

GLY

VAL

GLY

LEU

PHE

ARG

THR

GLU

PHE

5

LEU

TYR

MET

ASP

ARG

ASN

SER

LEU

PRO

SER

6

GLU

GLN

PHE

GLU

ALA

TYR

LYS

GLU

7

VAL

GLU

LYS

MET

GLY

ARG

PRO

VAL

8

THR

ILE

ARG

THR

LEU

ASP

ILE

GLY

ASP

9

LYS

GLU

LEU

PRO

TYR

LEU

ASP

MET

PRO

LYS

10

GLU

MET

ASN

PRO

PHE

LEU

GLY

TYR

ARG

ALA

11

ILE

ARG

LEU

CYS

LEU

ASP

ARG

PRO

ASP

ILE

12

PHE

LYS

THR

GLN

LEU

ARG

ALA

ILE

LEU

ARG

13

ALA

SER

ALA

TYR

GLY

ASN

VAL

GLN

ILE

MET

14

TYR

PRO

MET

ILE

SER

VAL

GLU

VAL

15

ARG

LYS

ALA

ASN

SER

ILE

LEU

GLU

VAL

16

LYS

ALA

GLU

LEU

ASP

ARG

GLU

GLY

VAL

LYS

17

TYR

ASP

LYS

GLU

ILE

LYS

VAL

GLY

ILE

MET

18

VAL

GLU

ILE

PRO

SER

ALA

VAL

THR

ALA

19

ASP

ILE

LEU

ALA

LYS

GLU

VAL

ASP

PHE

20

SER

ILE

GLY

THR

ASN

ASP

LEU

THR

GLN

TYR

21

THR

LEU

ALA

VAL

ASP

ARG

MET

ASN

GLU

HIS

22

VAL

LYS

GLU

TYR

GLN

PRO

PHE

HIS

PRO

23

ALA

ILE

LEU

ARG

LEU

VAL

LYS

MET

VAL

ILE

24

ASP

ALA

HIS

LYS

GLU

GLY

LYS

PHE

ALA

25

ALA

MET

CYS

GLY

GLU

MET

ALA

GLY

ASP

PRO

26

LEU

ALA

VAL

ILE

LEU

GLY

LEU

GLY

27

LEU

ASP

GLU

PHE

SER

MET

SER

ALA

THR

SER

28

ILE

PRO

GLU

ILE

LYS

ASN

ILE

ARG

ASN

29

VAL

GLU

TYR

GLU

LYS

ALA

LYS

GLU

ILE

ALA

30

GLU

LYS

ALA

LEU

ASN

MET

SER

GLU

ALA

ARG

31

GLU

ILE

GLU

LYS

MET

LYS

ASP

VAL

ILE

32

LYS

ASP

ILE

GLY

Samples:

sample_1: EIC from from Thermoanaerobacter Tengcongensis, [U-100% 13C; U-100% 15N], 0.5-1 mM; sodium chloride 100 mM; TRIS 20 mM; Magnesium Chloride 4 mM; EDTA 1 mM

sample_conditions_1: pH: 7.4; temperature: 343 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks