BMRB Entry 27179

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Hepatitis B Virus X protein (HBx)
Deposition date:
2017-07-14
Original release date:
2019-03-15
Authors:
Delgado, Leonildo; Grzesiek, Stephan
Citation:

Citation: Delgado, Leonildo; Grzesiek, Stephan; Delgado, Leonildo; Grzesiek, Stephan. "1H, 15N, 13C assignments and 15N relaxation data of the intrinsically disordered hepatitis B virus X protein (HBx) in a detergent mixture"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Hepatitis_B_virus_X_protein_(HBx), polymer, 177 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pROX180-9CS N-ter

Data typeCount
13C chemical shifts450
15N chemical shifts115
1H chemical shifts629
T1 relaxation values219
T2 relaxation values219
heteronuclear NOE values224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hepatitis B virus X protein (HBx)1

Entities:

Entity 1, Hepatitis B virus X protein (HBx) 177 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYSERGLYSERALAALAARGLEUSERSER
4   GLNLEUASPPROALAARGASPVALLEUSER
5   LEUARGPROVALGLYALAGLUSERARGGLY
6   ARGPROPHESERGLYSERLEUGLYTHRLEU
7   SERSERPROSERPROSERALAVALPROTHR
8   ASPHISGLYALAHISLEUSERLEUARGGLY
9   LEUPROVALSERALAPHESERSERALAGLY
10   PROSERALALEUARGPHETHRSERALAARG
11   ARGMETGLUTHRTHRVALASNALAHISGLN
12   ILELEUPROLYSVALLEUHISLYSARGTHR
13   LEUGLYLEUSERALAMETSERTHRTHRASP
14   LEUGLUALATYRPHELYSASPSERLEUPHE
15   LYSASPTRPGLUGLULEUGLYGLUGLUILE
16   ARGLEULYSVALPHEVALLEUGLYGLYSER
17   ARGHISLYSLEUVALSERALAPROALAPRO
18   SERASNPHEPHETHRSERALA

Samples:

sample_1: Hepatitis B virus X protein (HBx), [U-100% 13C; U-100% 15N], 0.7 mM; sodium acetate 20 mM; EDTA 2 mM; DHPC 100 mM; Fos-Choline-12 2.8 mM; sodium chloride 0 mM

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
4D (H)NNH)sample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

PIPP, Garrett - peak picking

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks