BMRB Entry 27173

Name: 1H, 13C, and 15N resonance assignments of a 22mer G-quadruplex forming within KRAS oncogene promoter region at physiological temperature
Published: 2017-07-20

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PDB ID: 6t51
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27173
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of a 22mer G-quadruplex forming within KRAS oncogene promoter region at physiological temperature

Deposition date:

2017-07-12

Original release date:

2017-07-20

Authors:

Marquevielle, Julien; Kumar M.V., Vasantha; Mergny, Jean-Louis; Salgado, Gilmar F.

Citation:

Citation: Marquevielle, Julien; Kumar M.V., Vasantha; Mergny, Jean-Louis; Salgado, Gilmar F.. "1H, 13C, and 15N chemical shift assignments of a G-quadruplex forming sequence within the KRAS proto-oncogene promoter region" Biomol. NMR Assignments 12, 123-127 (2018).
PubMed: 29189986

Assembly members:

Assembly members:
KRAS22RT, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KRAS22RT: AGGGCGGTGTGGGAATAGGG AA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	136
15N chemical shifts	12
1H chemical shifts	203

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KRAS promoter region G-quadruplex	1

Entities:

Entity 1, KRAS promoter region G-quadruplex 22 residues - Formula weight is not available

1

DA

DG

DC

DG

DT

DG

DT

2

DG

DA

DT

DA

DG

3

DA

Samples:

sample_1: KRAS22RT 2.5 mM

Buffer_1X: pH: 6.66; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	Buffer_1X
2D 1H-13C HSQC	sample_1	isotropic	Buffer_1X
2D 1H-13C HSQC aromatic	sample_1	isotropic	Buffer_1X

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 950 MHz
Bruker Avance 800 MHz
Bruker Avance 700 MHz