BMRB Entry 27172

Name: Ubiquitin V26A Mutant at pH 5.0
Published: 2017-08-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27172

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Ubiquitin V26A Mutant at pH 5.0

Deposition date:

2017-07-11

Original release date:

2017-08-04

Authors:

Bruett, Carter; Soss, Sarah; Flynn, Peter

Citation:

Citation: Bruett, Carter; Soss, Sarah; Flynn, Peter; Bruett, Carter; Soss, Sarah; Flynn, Peter. "Ubiquitin mutant V26A pH 5.0" The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
V26A_Ubiquitin, polymer, 82 residues, 9359 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
V26A_Ubiquitin: MQIFVKTLTGKTITLEVEPS DTIENAKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGHHHH HH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	220
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ubiquitin	1

Entities:

Entity 1, Ubiquitin 82 residues - 9359 Da.

1

MET

GLN

ILE

PHE

VAL

LYS

THR

LEU

THR

GLY

2

LYS

THR

ILE

THR

LEU

GLU

VAL

GLU

PRO

SER

3

ASP

THR

ILE

GLU

ASN

ALA

LYS

ALA

LYS

ILE

4

GLN

ASP

LYS

GLU

GLY

ILE

PRO

ASP

GLN

5

GLN

ARG

LEU

ILE

PHE

ALA

GLY

LYS

GLN

LEU

6

GLU

ASP

GLY

ARG

THR

LEU

SER

ASP

TYR

ASN

7

ILE

GLN

LYS

GLU

SER

THR

LEU

HIS

LEU

VAL

8

LEU

ARG

LEU

ARG

GLY

HIS

9

HIS

Samples:

sample_1: V26A Ubiquitin, [U-99% 13C; U-99% 15N], .337 mM; D2O, [U-99% 2H], 10 v/v; H2O 90 v/v; sodium azide 0 na; acetic acid 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ v4.2 Rev A, Varian - collection

Felix v2007, Accelrys Software Inc. - processing

SPARKY v3.115, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Agilent INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks