BMRB Entry 27171

Name: FXN_AIN_ISCU
Published: 2017-07-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27171

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FXN_AIN_ISCU

Deposition date:

2017-07-10

Original release date:

2017-07-12

Authors:

Cai, Kai; Markley, John

Citation:

Citation: Cai, Kai; Frederick, Ronnie; Tonelli, Marco; Markley, John. "Interactions of iron-bound frataxin with ISCU and ferredoxin on the cysteine desulfurase complex leading to Fe-S cluster assembly." J. Inorg. Biochem. 183, 107-116 (2018).
PubMed: 29576242

Assembly members:

Assembly members:
Frataxin, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Frataxin: SGTLGHPGSLDETTYERLAE ETLDSLAEFFEDLADKPYTF EDYDVSFGSGVLTVKLGGDL GTYVINKQTPNKQIWLSSPS SGPKRYDWTGKNWVFSHDGV SLHELLAAELTKALKTKLDL SWLAYSGKDA

Data sets:

spectral_peak_list

Data type	Count
.	.

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Frataxin Mature Version	1

Entities:

Entity 1, Frataxin Mature Version 130 residues - Formula weight is not available

1	SER	GLY	THR	LEU	GLY	HIS	PRO	GLY	SER	LEU
2	ASP	GLU	THR	THR	TYR	GLU	ARG	LEU	ALA	GLU
3	GLU	THR	LEU	ASP	SER	LEU	ALA	GLU	PHE	PHE
4	GLU	ASP	LEU	ALA	ASP	LYS	PRO	TYR	THR	PHE
5	GLU	ASP	TYR	ASP	VAL	SER	PHE	GLY	SER	GLY
6	VAL	LEU	THR	VAL	LYS	LEU	GLY	GLY	ASP	LEU
7	GLY	THR	TYR	VAL	ILE	ASN	LYS	GLN	THR	PRO
8	ASN	LYS	GLN	ILE	TRP	LEU	SER	SER	PRO	SER
9	SER	GLY	PRO	LYS	ARG	TYR	ASP	TRP	THR	GLY
10	LYS	ASN	TRP	VAL	PHE	SER	HIS	ASP	GLY	VAL
11	SER	LEU	HIS	GLU	LEU	LEU	ALA	ALA	GLU	LEU
12	THR	LYS	ALA	LEU	LYS	THR	LYS	LEU	ASP	LEU
13	SER	TRP	LEU	ALA	TYR	SER	GLY	LYS	ASP	ALA

Samples:

sample_1: Frataxin, [U-100% 15N], 0.3 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 750 MHz

1	SER	GLY	THR	LEU	GLY	HIS	PRO	GLY	SER	LEU
2	ASP	GLU	THR	THR	TYR	GLU	ARG	LEU	ALA	GLU
3	GLU	THR	LEU	ASP	SER	LEU	ALA	GLU	PHE	PHE
4	GLU	ASP	LEU	ALA	ASP	LYS	PRO	TYR	THR	PHE
5	GLU	ASP	TYR	ASP	VAL	SER	PHE	GLY	SER	GLY
6	VAL	LEU	THR	VAL	LYS	LEU	GLY	GLY	ASP	LEU
7	GLY	THR	TYR	VAL	ILE	ASN	LYS	GLN	THR	PRO
8	ASN	LYS	GLN	ILE	TRP	LEU	SER	SER	PRO	SER
9	SER	GLY	PRO	LYS	ARG	TYR	ASP	TRP	THR	GLY
10	LYS	ASN	TRP	VAL	PHE	SER	HIS	ASP	GLY	VAL
11	SER	LEU	HIS	GLU	LEU	LEU	ALA	ALA	GLU	LEU
12	THR	LYS	ALA	LEU	LYS	THR	LYS	LEU	ASP	LEU
13	SER	TRP	LEU	ALA	TYR	SER	GLY	LYS	ASP	ALA

1	SER	GLY	THR	LEU	GLY	HIS	PRO	GLY	SER	LEU
2	ASP	GLU	THR	THR	TYR	GLU	ARG	LEU	ALA	GLU
3	GLU	THR	LEU	ASP	SER	LEU	ALA	GLU	PHE	PHE
4	GLU	ASP	LEU	ALA	ASP	LYS	PRO	TYR	THR	PHE
5	GLU	ASP	TYR	ASP	VAL	SER	PHE	GLY	SER	GLY
6	VAL	LEU	THR	VAL	LYS	LEU	GLY	GLY	ASP	LEU
7	GLY	THR	TYR	VAL	ILE	ASN	LYS	GLN	THR	PRO
8	ASN	LYS	GLN	ILE	TRP	LEU	SER	SER	PRO	SER
9	SER	GLY	PRO	LYS	ARG	TYR	ASP	TRP	THR	GLY
10	LYS	ASN	TRP	VAL	PHE	SER	HIS	ASP	GLY	VAL
11	SER	LEU	HIS	GLU	LEU	LEU	ALA	ALA	GLU	LEU
12	THR	LYS	ALA	LEU	LYS	THR	LYS	LEU	ASP	LEU
13	SER	TRP	LEU	ALA	TYR	SER	GLY	LYS	ASP	ALA

1	SER	GLY	THR	LEU	GLY	HIS	PRO	GLY	SER	LEU
2	ASP	GLU	THR	THR	TYR	GLU	ARG	LEU	ALA	GLU
3	GLU	THR	LEU	ASP	SER	LEU	ALA	GLU	PHE	PHE
4	GLU	ASP	LEU	ALA	ASP	LYS	PRO	TYR	THR	PHE
5	GLU	ASP	TYR	ASP	VAL	SER	PHE	GLY	SER	GLY
6	VAL	LEU	THR	VAL	LYS	LEU	GLY	GLY	ASP	LEU
7	GLY	THR	TYR	VAL	ILE	ASN	LYS	GLN	THR	PRO
8	ASN	LYS	GLN	ILE	TRP	LEU	SER	SER	PRO	SER
9	SER	GLY	PRO	LYS	ARG	TYR	ASP	TRP	THR	GLY
10	LYS	ASN	TRP	VAL	PHE	SER	HIS	ASP	GLY	VAL
11	SER	LEU	HIS	GLU	LEU	LEU	ALA	ALA	GLU	LEU
12	THR	LYS	ALA	LEU	LYS	THR	LYS	LEU	ASP	LEU
13	SER	TRP	LEU	ALA	TYR	SER	GLY	LYS	ASP	ALA